Study on the function of chemokine receptor CCR7 in metastasis of stom-ach cancer
- VernacularTitle:趋化因子受体CCR7在胃癌转移中的作用研究
- Author:
Huan WANG
1
;
Wufeng YUAN
;
Lei JIANG
;
Xianming FEI
Author Information
1. 浙江省人民医院检验中心
- Keywords:
Chemokine receptor CCR7;
pLVX-Puro plasmid;
SGC7901 cell;
Stomach cancer;
Metastasis
- From:
China Modern Doctor
2015;(15):14-16,161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct stable expression system of pLVX-Pro-CCR7 and study the function of CCR7 gene in metastasis of stomach cancer. Methods PCR method was used to amplify CCR7 gene and construct pLVX-Puro-CCR7 recombinant plasmid. Lipofection was used to infect SGC7901 cell strain immediately, and puromycin was used to screen the cell strain and establish a cell strain with stable expression. Western blot method was used to test the ex-pression of CCR7 in SGC7901 cells which were stably infected. Transwell method was used to analyze the effect of overly expressed CCR7 gene on in vitro migration of stomach cancer cells of SGC7901. Living imaging was used to ob-serve the metastasis ability of stomach cancer cell strain of SGC7901 which overly expressed CCR7 in nude mice. Re-sults pLVX-Puro-CCR7 recombinant plasmid was successfully constructed and SGC791 cell strain was stably infected. The cell strain was able to correctly translate and express CCR7 protein. Metastasis ability in nude mice and in vitro migration ability of SGC7901 stomach cancer cell strain which overly expressed CCR7 gene significantly improved (P<0.05). Conclusion CCR7 may be involved in the function of promoting metastasis of stomach cancer cells.
