Effects of induced differentiation and micro-capsule on the secretion ca-pacity of insulin-producing cells
- VernacularTitle:微囊化胰岛素产生细胞的胰岛素释放情况观察
- Author:
Yaguang WANG
1
;
He TIAN
;
Changzheng MU
Author Information
1. 辽宁医学院组织学与胚胎学教研室
- Keywords:
Bone marrow mesenchymal stem cells(BMMSCs);
Insulin-producing cells(IPCs);
Micro-capsule
- From:
China Modern Doctor
2015;(15):8-10,161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of micro-capsule on the secretion capacity of insulin-producing cells(IPCs). Methods Stem cells from originate mouse bone marrow mesenchymal were isolated,induced and purified. Rat pancre-atic extract(RPE)was extracted from pancreases of rats.BMMSCs were induced by rat pancreatic extract. The induced cells were randomly divided into micro-encapsulated group and non-micro-encapsulated group.The experiment of glu-cose stimulation was performed to detect the level of insulin,respectively, at different time points (1, 2, 3, 5, 10, 15, 20, 25, 30 day).Results The level of insulin secretion was increased after 1, 2, 3, 5 days of culture in micro-encapsu-lated IPCs and non-micro-encapsulated IPCs,but there were no significantly differences among the groups. The level of insulin secretion was declined in non-microencapsulated IPCs at 10 day,while there was no decreased in micro-en-capsulated IPCs until 20 days. Conclusion The micro-capsule can promote the effect duration of IPCs,which supports the function of IPCs.
