Effects and mechanism of implantation of umbilical blood mesenchymal stem cells on rats with cerebral infarction
- VernacularTitle:脐血间充质干细胞移植对脑梗死大鼠的影响及作用机制
- Author:
Hong SHAN
1
;
Jianbin LI
;
Min LIU
;
Dongpeng LI
;
Jiaojie WANG
;
Huitao LIANG
;
Zheng QI
Author Information
1. 河南省红十字血液中心
- Keywords:
Umbilical blood mesenchymal stem cells(MSCs);
Cerebral infarction;
Neuroplasticity
- From:
China Modern Doctor
2015;(14):21-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and mechanism of implantation of umbilical blood mesenchymal stem cells(MSCs)on rats with cerebral infarction. Methods Umbilical blood MSCs were cultured in vivo,flow cytometry was ap-plied to test cytometric immunophenotype, and model of rat cerebral infarction was made by suture method; 120 rats were randomly assigned to MSCs implantation group and control group; rats' neurological function was evaluated;western-blot was applied to test the expression of GFAP protein. Fluorescence microscope was applied to observe changes of hippocampal formation in rats as well as the distribution of MSCs in rats' brain. Results According to the test of neurological function, the score of neurological function in the experiment group was reduced, and the score in the control group was improved. The difference was statistically significant(P<0.05). Expression of GFAP in MSCs im-plantation group was increased with a peak in the 7th day,while no significant changes in the control group(P<0.05). According to the observation of immunofluorescence microscopy, the distribution of MSCs in rats' brain was good in MSCs implantation group, and the hippocampal formation showed clear layers in the experiment group. Hippocampal structures in the control group were chaotic, and synapses and organelles were dissolved and compromised. Conclu-sion MSCs are able to promote the repair of hippocampal structures after cerebral infarction in rats, strengthen neuro-plasticity and promote the recovery of neurological functions.
