The expression of connective tissue growth factor and integrin β1 in vagi-nal anterior wall of the model rats with stress urinary incontinence
- VernacularTitle:压力性尿失禁大鼠模型阴道前壁组织中结缔组织生长因子和整合素β1的表达
- Author:
Can CUI
1
;
Zhilian WANG
;
Min HAO
;
Xiaoxia SUN
Author Information
1. 山西医科大学第二医院妇产科
- Keywords:
Stress urinary incontinence;
Connectivetissue growth factor;
Integrinβ1
- From:
China Modern Doctor
2015;(11):25-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of the connective tissue growth factor (CTGF) and integrin β1 in vaginal anterior wall of the model rats with stress urinary incontinence. Methods Twenty-four healthy female SD rats were randomly divided into three groups, group A rats as control group without any treatment;group B rats only with vaginal expansion;group C rats with vaginal expansion and bilateral ovaries removed. At postoperative four weeks to determine the maximum bladder capacity (MBC)and abdominal pressure leakage point pressure (ALPP), to establish detection model for stress urinary incontinence. Modeling successfully executed in rats after four weeks after vaginal anterior wall tissue,immunohistochemical staining method and real-time fluorescent quantitative RT-PCR technique to detect the expression of CTGF and integrinβ1. Results The urine flow dynamic detection(MBC,ALPP)B and C group was significantly lower than group A, there were statistically significant differences (P<0.05);CTGF mRNA and protein expression in group C was significantly lower than group A(P<0.05),there were no statistical significance between group A and group B (P>0 . 05);integrin β1 mRNA and protein in group C expression was low er than that in group A and B(P<0.05), and differences between A and B were statistically significant(P<0.05). Conclusion The anterior wall vaginal CTGF in rats in SUI and low expression of integrin β1 may be related to stress urinary incontinence and other pelvic floor support structure there is a close relationship of the occurrence of degenerative diseases of the pelvic floor support degenerative diseases.
