COL1A1 promoter polymorphisms analysis by pyrosequencing and sus-ceptibility to osteosarcoma
- VernacularTitle:焦磷酸测序用于COL1A1基因多态性与骨肉瘤易感性关系分析
- Author:
Maohua YAN
1
;
Bin XU
;
Lilai ZHAO
;
Qiuliang ZHU
;
Jianmin LUO
;
Zhengming YANG
Author Information
1. 浙江省安吉县人民医院骨科
- Keywords:
Osteosarcoma;
CollagenⅠ;
Gene polymorphism
- From:
China Modern Doctor
2015;(3):19-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlationship between type Ⅰcollagenα1 (COL1A1) gene polymorphism and the occurrence of osteosarcoma. Methods Peripheral blood from 54 patients with osteosarcoma and 126 normal ones were collected, rs1061970 genotype of COL1A1 gene was amplified with PCR and products were analyzed by pyrosequencing among the samples. Results The allele frequency of TT (13.0%) and CT (48.1%) was significantly higher in pathologi-cal group than that in the normal control group, which manifested a allele frequency of TT(11.9%) and CT(30.2%) (P<0.05). Additionally, allele frequency of T in patients with osteosarcoma was 37.0%, higher than the control group (27.0%), with OR of 1.59 and 0.99-2.57 of 95%CI, with no difference of statistically significant (P>0.05), but the risk was still on the rise of osteosarcoma. Conclusion COL1A1 gene polymorphism may be related with the incidence of osteosarcoma, patients who carry the T allele of gene of COL1A1 may increase the risk of osteosarcoma occurrence.
