Expression and clinical significance of miR-196a in plasma from hy-popharyngeal squamous cell carcinoma patients
- VernacularTitle:miR-196a在下咽鳞状细胞癌血浆中的表达及其临床意义
- Author:
Zhenhua WU
1
;
Qun LI
;
Xiang CUI
;
Zhisen SHEN
Author Information
1. 宁波市医疗中心李惠利医院耳鼻咽喉头颈外科
- Keywords:
miRNA;
Hypopharyngeal cancer;
Biomarker;
Diagnosis
- From:
China Modern Doctor
2015;(3):11-15,161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the expression level of miR-196a in plasma and the clin-icopathologic factors of HSCC. Methods Paired plasma specimens from HSCC patients and 50 healthy people were collected. Plasma total RNA was extracted from 57 HSCC patients and 50 healthy people ,and the expression level of miR-196a was measured by quantitative real time PCR. The clinicopathological features between the plasma expression level of miR-196a were analyzed. ROC curve were constructed to evaluate the diagnostic value. Results miR-196a exhi-biting higher expression in pre-operation HSCC plasma with △Ct=9.11(5.69,12.43)compared with post-operation HSCC plasma with△Ct=12.69(9.16,14.84)and healthy group with△Ct=12.85(11.18,14.91)(P<0.01). There were no significant differences between the expression level of miR-196a in healthy group and post-operation group (P>0.05). miR-196a levels in plasma were correlated with grade of differentiation (P<0.05), lymph node metastasis(P<0.01), and clinical stage(P<0.05), while there was no relationship with age and smoking history(P>0.05). The areas under the ROC curves were 0.755. The cut-off point of△Ct was 10.84,with the 95%CI=0.66-8.85(P<0.01). Conclusion Plasma expression level of miR-196a have realationship with clinical stage ,lymph node matastasis and grade of differentiation. miR-196a may have involved in carcinogenesis and tumor development of HSCC. Plasma miR-196a could be potential minimally invasive biomarkers for HSCC patients.
