Experimental study of Mg 2+on effects of cell apoptosis of rabbit secondary brain injury
- VernacularTitle:Mg2+对兔脑继发性脑损伤中细胞凋亡影响的实验研究
- Author:
Qihui ZHANG
1
;
Shijie DONG
;
Zhidi LI
;
Huaxiao LI
;
Shiqiang WANG
Author Information
1. 广州医科大学附属深圳沙井医院神经外科
- Keywords:
Experimental study;
Cell apoptosis;
Secondary brain injury;
Mg 2+
- From:
China Modern Doctor
2014;(35):5-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the experiment of Mg2+on effects of cell apoptosis of rabbit secondary brain injury. Methods Fifty New Zealand white rabbits, irrespective of genders were selected, they were randomly divided into ex-perimental group, control group and operation control group, 20 rats in each group of the experimental group and the control group, 10 rabbits were in the sham operation group; animal were used magnesium sulfate after brain injury in the early vein,through the pathology examination of brain tissue around contusion foci, X-ray examination of electron microscopy and cell apoptosis detection, the effect of magnesium ion on the cell apoptosis in secondary brain damage was analyzed. Results The control group after 6 h of brain injury, pathology showed focus of the lesions appeared haem-orrhage, the organization gap widened, peripheral edema, 48 h after brain injury was most significant, the lesions ap-peared cell membrane structure complete, the nucleus pycnosised nerve cells,that was to say, neural cell apoptosis, apoptotic cells were relatively dense; Neural cell degeneration and necrosis of experimental group were less , brain edema was lighter, there were no significant differences on both sides of the lesions; the expression of TUNEL positive cells in normal brain tissue was less, 6-hour-injury specimens appeared TUNEL powder in a small number of positive cells, the number of TUNEL positive cells of specimens of two groups increased with the prolongation of injury time, the peak period was 24 hours, TUNEL positive cells at different stages in the experimental group were significantly lower than those of the control group at the same time segment, the difference had statistical significance (P<0.05). Conclusion Magnesium ions can reduce the number of nerve cell apoptosis of rabbit secondary brain injury, play a pro-tective role in injury of cerebral nerve cells.
