Influence of pioglitazone on tissue TNF-α,IL-10 and cell apoptosis with cerebral ischemia-reperfusion injury in rats
- VernacularTitle:吡格列酮对急性脑缺血再灌注损伤大鼠TNF-α、IL-10和细胞凋亡的影响
- Author:
Yu ZHAO
1
;
Zhi YU
;
Boda LU
;
Kaide LIU
;
Laiming FANG
Author Information
1. 浙江省诸暨市第六人民医院内科
- Keywords:
Pioglitazone;
Cerebral ischemia reperfusion;
TNF-α;
IL-10;
Cell apoptosis
- From:
China Modern Doctor
2014;(35):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of TNF-α, IL-10 and cell apoptosis of cerebral ischemia-reperfusion injury and the influence of Pioglitazone(PGZ) on these parameters in rats. Methods Eighty-four male SD rats were ran-domly divided into two groups, PGZ group (n=42) and the control group (n=42). The PGZ group was treated with improved Zea-longa method and received tail vein injections of PGZ (10 mg/kg) immediately after injury, the control group re-ceived tail vein injections with the same dose sodium chloride injection immediately, after injury and repeat one time everyday until the rats was killed. Each group was divided into seven subgroups by sacrificed time after injury, those were 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, and 7 d, each subgroup got 6 rats. Each subgroup were randomly selected three rats after being killed, detected the expression of TNF-α and IL-10 of rats contusion peri tissues brain tissue by using im munohistochemical methods, while using TUNEL method to observe the peri cell apoptosis after brain contusion. Results The expression of TNF-α in each PGZ group was significantly decreased but the IL-10 was significant ly increased compared with the control group (P<0.05),and a significant negative correlation between the both of parameters in two groups as well (P<0.01);At the same time the number of apoptotic cells was decreasing (P<0.05). Conclusion Pioglita-zone is probably through the route of relieving inflammation response, reducing the change of secondary brain injury af-ter traumatic brain injury and decreasing neural cell apoptosis, and then provide protection of neurocytes.
