The effect of roxithromycin on expressions of HDAC2 in smoking asthma mice
- VernacularTitle:罗红霉素对香烟烟雾暴露小鼠HDAC2的影响
- Author:
Hui XU
1
;
Yuanrong DAI
;
Danhong HU
;
Mengling XIA
Author Information
1. 温州医科大学附属第二医院呼吸科
- Keywords:
Asthma;
Cigarette smoke;
Histone Deacetylases;
Roxithromycin
- From:
China Modern Doctor
2014;(30):1-3,6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of roxithromycin on airway inflammation of smoking asthma though re-searching its effect on expressions of histone deacetylase-2(HDAC2)of cigarette smoking asthma mice. Methods Forty female SPF BALB/c mice were divided randomly into 4 groupscontrol group(C),asthma group(A),smoking asthma group(S)and Roxithromycin group(R). Established asthmatic and smoking asthma models with challenge of OVA and intervention with Roxithromycin. The change of pathematology in different groups were observed. The different cells counts of bronchoalveolar fluid (BALF) were analyzed. The level of HDAC2 in lung homogenate were measured by Western blot. Results The ratios of eosinophil (EOS) to the total cell numbers of BALF in group A and group S were significantly higher than that in group C (both P<0.01), while in group S the ratio of neutrophile (NEU) was higher than the group A (P<0.01). The ratios of EOS and NEU in group R was significantly decreased than these in group S(P<0.01). Western blot showed expressions of HDAC2 in group A and group S were significantly decreased than that in group C(both P<0.01),while the HDAC2 level of group S was lower than that of group A(P<0.01). The HDAC2 level of group R was higher than group S(P<0.01). Conclusion Roxithromycin can decrease the airway inflammation of cigarette smoking asthma mice via increasing the expression of HDAC2.
