The function of transcription factor on treatment of montelukast sodium tables for patients with steroid-resistant allergic rhinitis
- VernacularTitle:转录因子在孟鲁司特钠治疗类固醇激素抵抗型变应性鼻炎中的作用
- Author:
Gang REN
1
;
Jue XU
;
Xudong LUO
;
Longjiang LAN
;
Manqun LI
;
Bingliang MA
;
Jianqiu WANG
Author Information
1. 浙江省湖州市第一人民医院 湖州师范学院附属第一医院耳鼻咽喉科
- Keywords:
Perennial allergic rhinitis;
Steroid-resistant;
Activator protein-1;
Nuclear factor-κB;
Interleukin-4;
Inter-leukin-5;
Interleukin-10
- From:
China Modern Doctor
2014;(26):41-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of activator protein-1(AP-1)and nuclear factor-κB(NF-κB)on the treatment of montelukast sodium tables for patients with steroid-resistant allergic rhinitis (AR). Methods A random-ized study was carried out in 50 patients with steroid-resistant AR. 25 cases were treated with montelukast sodium ta-bles(montelukast sodium group), and the others by ATP tables(placebo group). Score system was used to compare the therapeutic effect of these two drugs on clinical symptoms and signs on 21th day after therapy. The DNA binding activ-ities of Ap-1 and NF-κB were measured by electrophoretic mobility shift assay(EMSA). The expression of IL-4, IL-5 and IL-10 were determined by enzyme-linked immunoadsorbent assay (ELISA). Results The therapeutic effect rate in montelukast sodium group was significantly higher than that in placebo group at 21 days after treatment begining (P<0.01). Compared with placebo group, the DNA binding activities of Ap-1 and NF-κB were significantly reduced in montelukast sodium group (P<0.01). The score reduce index (SRI)of the clinical symptoms and signs was negatively correlated with the DNA binding activities in montelukast sodium group (P<0.05), but there was any correlation be-tween them in placebo group(P>0.05).The expression of IL-4 and IL-5 in montelukast sodium group were significantly lower and IL-10 was higher than those in placebo group (P<0.01). Conclusion The treatment effect of montelukast sodium tables for steroid-resistant AR may be aroused by suppressing the Ap-1 and NF-κB DNA binding activities, and effecting the expression of IL-4, IL-5 and IL-10.
