The mechanism of miR-26a inhibiting gastric cancer cells proliferation
- VernacularTitle:miR-26a抑制胃癌AGS细胞生长的分子机制
- Author:
Fabao SHAO
1
;
Zhuli XU
;
Qinlan HUANG
Author Information
1. 广东医学院附属厚街医院内科
- Keywords:
miR-26a;
Gastric cancer;
Growth and proliferation
- From:
China Modern Doctor
2014;(26):11-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explicit the expression and role of miR-26a in gastric cancer tissues, thus to reveal molecular mechanism that miR-26a functions in gastric cancer cells. Methods The qRT-PCR was conducted for detect the ex-pression of miR-26a in 71 cases of gastric cancers. AGS cells were transfected with miR-26a mimics, then Western blot was performed to detect the expressions of MTDH protein. The proliferation ability of AGS cells evaluated by MTT assy. Results qRF-PCR showed that miR-26a was down-regulated 0.44 times in 71 cases of gastric cancer tissues. Western blot showed that the expressions of MTDH protein was inhibited by restored miR-26a or MTDH siRNA in AGS cells. Overexpression of miR-26a or MTDH siRNA inhibited the proliferation of AGS cells. Their OD values of transfected miR-26a mimics and MTDH siRNA were (0.158±0.006),(0.201±0.006) respectively, and their OD values of transfected control were (0.515±0.032),(0.479±0.028) respectively,there were statistically significance(P<0.05). Con-clusion miR-26a suppresses cell proliferation by targeting MTDH in gastric cancer.
