Prokaryotic expression of coxsackievirus group A type 16 VP1 protein
- VernacularTitle:柯萨奇病毒A组16型VP1蛋白的原核表达
- Author:
Zhihong WANG
1
;
Yingying DING
;
Jiaojiao FENG
;
Lianqing LI
;
Wei PAN
;
Cunjiu GUO
Author Information
1. 山西医科大学第二附属医院检验科
- Keywords:
Coxsackievirus group A type 16(CA16);
VP1 protein;
Peokaryotic expression;
Enzyme-linked immunosor-bent assay
- From:
China Modern Doctor
2014;(14):69-72,75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the recombinant plasmid of coxsackievirus group A type 16 (CA16)VP1,express in E.coli and identify expressed product. Methods The CA16 VP1 gene was amplified by PCR,The constructed recombi-nant plasmid pET21b-CA16 VP1 was transformed into E.coli BL21(DE3) for expession and purification,expressed and purified product was identified by SDS-PAGE,Indirect ELISA tested Antigenicity and validity of recombinant proteins CA16 VP1. Results The coxsackievirus group A type 16 VP1 protein was expressed in peokaryotic cells and purified, which showed specific reaction with mice antiserum against CA16 virus,high reactivity with part serums of the elderly population in Taiyuan city. Conclusion CA16 VP1 protein was successfully expressed, had good Antigenicity and va-lidity,which will lay a foundation for study on the CA16 diagnostic kits.
