The Effects of UTI on Brain Edema and CD11b in Brains of Rats after CPR
10.3969/j.issn.1673-9701.2009.17.012
- VernacularTitle:乌司他丁对心肺复苏后大鼠脑组织含水量及CD11b的影响
- Author:
Xiaofeng ZHOU
1
;
Jing WANG
;
Yan ZHAO
;
Xiurong XING
;
Jian QIN
Author Information
1. 首都医科大学大兴医院
- Keywords:
Cardiopulmonary resuscitation;
Isehemical reperfusion injury;
Brain edema;
CD11b;
Uiinastatin(UTI)
- From:
China Modern Doctor
2009;47(17):25-26,37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of UTI on the moisture capacity and CD11b of brains in CPR rats. Methods 30 grown male Sprague Dawley(SD) rats were divided randomly into three groups :control group,resuscitation group and UTI group. Each group had 10 rats. The last two groups were used to make CPR models induced by asphyxia and were observed lasting 2 hours after ROSC. Then all rats were caused dead and brain tissues were took out. Brain tissues were weighed (humid weight),then they were dried in a baker and scaled dry weight. Moisture capacities were calculated by Elliott method. The lever of CD11b in brain tissue was determined by ELISA method. Results ①The brain water content in the resuscitation group was significantly higher than that in the control group[(80.4 ± 2.0)% vs (76.7 ± 1.3)%, P < 0.01];The brain water content in UTI group was decreased significantly than that in the resuscitation group[(77.6 ± 1.5)% vs (80.4 ± 2.0)%, P<0.01]. ②The levels of CD11b of brain tissues in the resuscitation group increased significantly than those in the control group [(620.488 ± 38.723) ng/mg vs (453.189 ± 121.403) ng/mg, P < 0.01]; The levels of CD 11 b of brain tissues in UTI group decreased significantly than those in the resuscitation group[(476.875 ± 114.686)ng/mg vs (620.488 ± 38.723)ng/mg, P < 0.05]. Conclusion Brain edema occurs during the early stage of CPR,and the levers of CD11b in brain tissues increase significantly. The use of UTI may effectively reduce the brain water content,the levels of CD11b in brain tissues.
