Changes in Major Peanut Allergens Under Different pH Conditions.
10.4168/aair.2012.4.3.157
- Author:
Jihyun KIM
1
;
Jeongok LEE
;
Won Hee SEO
;
Youngshin HAN
;
Kangmo AHN
;
Sang Il LEE
Author Information
1. Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. snuhan@skku.edu
- Publication Type:Brief Communication
- Keywords:
Acetic acid;
allergens;
Ara h 1 allergen;
Ara h 2 allergen;
Ara h 3 allergen;
peanuts
- MeSH:
Acetic Acid;
Allergens;
Arachis;
Cooking;
Electrophoresis, Polyacrylamide Gel;
Food Habits;
Humans;
Hydrogen-Ion Concentration;
Immunoglobulin E;
Korea;
Peanut Hypersensitivity;
Prevalence
- From:Allergy, Asthma & Immunology Research
2012;4(3):157-160
- CountryRepublic of Korea
- Language:English
-
Abstract:
Regional dietary habits and cooking methods affect the prevalence of specific food allergies; therefore, we determined the effects of various pH conditions on major peanut allergens. Peanut kernels were soaked overnight in commercial vinegar (pH 2.3) or acetic acid solutions at pH 1.0, 3.0, or 5.0. Protein extracts from the sera of seven patients with peanut-specific IgE levels >15 kUA/L were analyzed by SDS-PAGE and immunolabeling. A densitometer was used to quantify and compare the allergenicity of each protein. The density of Ara h 1 was reduced by treatment with pH 1.0, 3.0, or 5.0 acetic acid, or commercial vinegar. Ara h 2 remained largely unchanged after treatment with pH 5.0 acetic acid, and was decreased following treatment with pH 1.0, 2.3, or 3.0 acetic acid. Ara h 3 and Ara h 6 appeared as a thick band after treatment with pH 1.0 acetic acid and commercial vinegar. IgE-binding intensities to Ara h 1, Ara h 2, and Ara h 3 were significantly reduced after treatment with pH 1.0 acetic acid or commercial vinegar. These data suggest that treatment with acetic acid at various pH values affects peanut allergenicity and may explain the low prevalence of peanut allergy in Korea.
