The Relationship between the Gene Polymorphism of Tumor Necrosis Factor-αPromoter Region in Hyperuricemia Patients and its Metabolic Phenotype
10.3969/j.issn.1673-9701.2008.01.001
- VernacularTitle:高尿酸血症TNF-α启动子区基因多态性与其代谢表型的关系
- Author:
Lanlan YAO
1
;
Xinyan CHEN
;
Feng XU
;
Jie PAN
;
Yangang WANG
Author Information
1. 青岛大学医学院附属医院
- Keywords:
Hyperuricemia;
Insulin;
Gene polymorphism;
Metabolic phenotype;
TNF-α
- From:
China Modern Doctor
2008;46(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between the-308 site,-238 site gene polymorphism of TNF-αa promoter region in hypemricemh and its metabolic phenotype.Methods HUA patients 188,normal people(NHU)51,the-308G/A site single nu-cleofide polymorphism of TNF-a gene promoter region was decided by Polymerase chain reaction-restriction fragment length polymor-phism(PCR-RFLP)method.The level of serum uric acid is detected by uricase.blood glucose by glucose oxidase.Selective inhibition assay for total cholesterol,triglyceride and high-density lipoprotein cholesterol.All these tests were complted within three hours afterextraction.Keep blood samples at-20℃ to detect the concentration of insulin using immunoradiometrie assay.HUA patients 35.NHU 39,the -238G/A site single nucleofide polymorphism of TNF-a gene promoter region was decided by the same method.Results By smdying two single nucleotide polymorphism,the GA+AA frequency of-308 site(23.94%)in HUA was significantly higher than NHU(11.76%)(P=0.0001);the genotype frequencies of GA and GG in-238 site had no significant difference between the two groups(P=0.08).Meanwhile,comparing GA+AA genotype and CG group of-308 site,waist-hip ratio,systolic and diastolic blood pressure,uric acid,triglycerides differences were statistically significant(P=0.05-0.01);the difference in-238 site between the two genotypes waft no significance.Conclusion The results of this study show-308A carriers of TNF-α promoter region in HUA individual is correlated with hyperuficemia and its metabolic phenotype.
