Effects of ligustilide on chemotherapy resistance of cervical cancer cells
- VernacularTitle:藁本内酯对宫颈癌细胞化疗耐药性的影响
- Author:
Wenyuan ZHANG
1
;
Qian WANG
1
;
Lihong ZHANG
1
;
Haiyan ZHOU
2
;
Ni ZHANG
3
Author Information
1. Dept. of Gynecology and Obstetrics，Qingyang Hospital of Traditional Chinese Medicine，Gansu Qingyang 745000，China
2. The 3rd Dept. of Obstetrics and Gynecology，Northwest Women and Children’s Hospital，Xi’an 710061，China
3. Genetics Teaching and Research Office，Basic Medicine Department，Xi’an Medical University，Xi’an 710061，China
- Publication Type:Journal Article
- Keywords:
ligustilide;
cervical cancer cells;
cisplatin;
chemotherapy resistance;
Hippo-YAP signaling pathway
- From:
China Pharmacy
2024;35(13):1582-1587
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of ligustilide on chemotherapy resistance of cervical cancer cells based on Hippo-Yes-associated protein （YAP） signaling pathway. METHODS Human cervical cancer cisplatin-resistant cells HeLa/DDP were divided into control group， cisplatin group （10 μmol/L cisplatin）， cisplatin+ligustilide low-， medium- and high-concentration groups （10 μmol/L cisplatin+25， 50， 100 μmol/L ligustilide）. The proliferation， apoptosis， migration and invasion of HeLa/DDP cells were all detected in each group. The mRNA expressions of YAP and transcriptional coactivator with PDZ binding motif （TAZ） as well as the protein expressions of YAP， TAZ， matrix metalloproteinase 2 （MMP2）， Ki67， cleaved-caspase-3 and caspase-3 were determined in HeLa/DDP cells. RESULTS Compared with control group， the inhibitory rate， apoptotic rate and cleaved- caspase-3/caspase-3 of cisplatin group were increased significantly； scratch healing rate， the number of invasive cells， the mRNA expressions of YAP and TAZ， and the protein expressions of YAP， TAZ， MMP2 and Ki67 were decreased significantly in cisplatin group （P＜0.05）. Compared with cisplatin group， the inhibitory rate of cell proliferation， apoptotic rate and cleaved-caspase-3/ caspase-3 were further increased in cisplatin+ligustilide low-， medium- and high-concentration groups， while scratch healing rate， the number of invasive cells， the mRNA expressions of YAP and TAZ， and the protein expressions of YAP， TAZ， MMP2 and Ki67 were further decreased， in a dose-dependent manner （P＜0.05）. CONCLUSIONS Ligustilide can increase the sensitivity of drug-resistant cervical cancer cells to cisplatin by inhibiting Hippo-YAP signaling pathway.
