The effects of LINC00092 on the proliferation , migration and invasion of glioma
10.19405/j.cnki.issn1000-1492.2023.11.016
- Author:
Jinliang Yang
1
,
2
;
Jia Li
3
;
Jing An
4
;
Yuqiao Xi
5
;
Lei Ye
6
;
Hongwei Cheng
6
Author Information
1. Dept of Neurosurgery, The First Afiliated Hospital of Anhui Medical University, Hefei 230022
2. Dept of Neurosurgery,Anhui Hanku Medical Diagnosis Technology Co. ,Ltd , Fuyang 236000
3. Emergency Surgery Trauma Center, Anhui Hanku Medical Diagnosis Technology Co. ,Ltd , Fuyang 236000
4. Outpatient Operating Room, Anhui Hanku Medical Diagnosis Technology Co. ,Ltd , Fuyang 236000
5. Dept of Quality, Anhui Hanku Medical Diagnosis Technology Co. ,Ltd , Fuyang 236000)
6. Dept of Neurosurgery, The First Afiliated Hospital of Anhui Medical University, Hefei 230022
- Publication Type:Journal Article
- Keywords:
glioma;
prognosis;
LINC00092;
IGF2BP1;
invasion
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1898-1903
- CountryChina
- Language:Chinese
-
Abstract:
Objective :o explore the effects of long non⁃coding RNA LINC00092 on the proliferation , migration and invasion of glioma.
Methods :Using the cancer genome atlas(TCGA) and genotype⁃tissue expression (GTEx) databases , this study analyzed the expression of LINC00092 in pan⁃carcinoma and its effect on the prognosis of glioma. In addition , LINC00092 overexpression plasmid was constructed to detect the effects of LINC00092 on proliferation , migration , invasion and apoptosis of glioma cells by cell function experiments , including CCK⁃8 assay , Transwell assay and flow cytometry. Finally , qRT⁃PCR and Western blot were used to detect the effect of overexpression of LINC00092 on the expression level of IGF2BP1 .
Results :The analysis of public databases revealed a widespread downregulation of LINC00092 in tumors , and its association with the development of glioblastoma multiforme (GBM) and low⁃grade glioma (LGG) . In vitro experiments demonstrated that overexpression of LINC00092 significantly reduced the proliferation , migration and invasion of glioma cells , while promoting apoptosis. Moreover, overexpression of LINC00092 led to a decrease in the expression levels of IGF2BP1 .
Conclusion :LINC00092 may inhibit glioma proliferation , migration and invasion by targeting IGF2BP1 , and promote glioma cell apoptosis.
- Full text:2024071017442831762LINC00092对神经胶质瘤增殖、迁移和侵袭的影响_杨金亮.pdf
