Genetic variation analysis of Human Papillomavirus 16 E4 and L2 in women with cervical infection in Xinjiang Uygur Autonomous Region
10.19405/j.cnki.issn1000-1492.2023.11.014
- Author:
Haozheng Cheng
1
;
Jing Tuo
1
;
Yangliu Dong
1
;
Le Wang
1
;
Xiangyi Zhe
2
;
Hongtao Li
2
;
Dongmei Li
1
;
Zemin Pan
1
Author Information
1. Dept of Biochemistry and Molecular Biology,Shihezi University School ofMedicine ,Key Laboratory ofXinjiang Endemic and Ethnic Diseases ,Shihezi 832002
2. Dept of Anatomy and Histology and Embryology,Shihezi University School of Medicine ,Shihezi 832002)
- Publication Type:Journal Article
- Keywords:
Human Papillomavirus 16;
gene variation;
cervical cancer;
E4 and L2 genes;
single nucleotide poly⁃ morphisms
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1884-1889
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the nucleotide variation and protein amino acid changes of E4 and L2 genes of
Human Papillomavirus 16 (HPV16) , and to analyze the evolutionary characteristics of HPV16 virus.
Methods : 40 HPV16 infection⁃positive cervical exfoliated cells samples and tissue cell samples were collected from hospital , viral DNA was extracted , Sanger sequencing perform in cervical exfoliated cells DNA and high⁃throughput sequencing technology sequenced in cervical tissues DNA for E4 and L2 genes of HPV16 , HPV16 E4 and L2 gene phylogenetic evolution trees were constructed , and variation of HPV16 E4 and L2 genes were analyzed.
Results :There were 72 HPV16 E4 variant samples with nucleotide variants (4 missense mutations and 7 synonymous mutations) at 10 sites , HPV16 L2 gene variants in 74 samples , and nucleotide variants (23 missense mutations and 18 synonymous mutations) at 40 sites. The variation frequency of T4177C , A4288C and A4654C in cervical cancer was significantly higher than that in non⁃cervical cancer, and the difference was statistically significant (P < 0. 05) .
Conclusion:① The main HPV16 virus strains in Xinjiang are European strains , and a few are Asian strains. ② The mutation frequency of T4177C , A4288C and A4654C in HPV16 L2 gene is higher than that in non⁃cervical cancer, and G4181A is related to the Asian strain.
- Full text:2024071017311234257新疆维吾尔自治区妇女宫颈感...型E4和L2的遗传变异分析_程豪政.pdf
