The effect of adeno⁃associated virus delivery of shRNA against EP3 receptors in the bilateral lateral parabrachial nucleus of rats on fever
10.19405/j.cnki.issn1000-1492.2023.11.012
- Author:
Tianhui He
1
;
Nanping Wang
1
;
Sihao Wu
2
;
Yanlin Wei
2
;
Jianhui Xu
1
;
Jie Zhang
1
Author Information
1. Key Laboratory of Thermoregulation and Inflammation of Sichuan Higher Education Institutes , Chengdu Medical College , Chengdu 610500
2. School of Clinical Medicine , Chengdu Medical College , Chengdu 610500
- Publication Type:Journal Article
- Keywords:
fever;
EP3 receptors;
lateral parabrachial nucleus;
lipopolysaccharide;
prostaglandin E2
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1872-1877
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of adeno⁃associated virus ( AAV) delivery of short hairpin RNA ( shRNA) against the Ptger3 gene in the lateral parabrachial nucleus (LPB) on the fever induced by microinjection of prostaglandin E2 (PGE2 ) into the LPB and the intraperitoneal injection of lipopolysaccharide (LPS) .
Methods:AAV2⁃shRNA⁃Ptger3(EP3) ⅣEGFP ( shRNA⁃EP3) and AAV2⁃ CMV⁃ EGFP ( shRNA⁃control) viruses were constructed and transfected the rat LPB by stereotaxic injection. Four weeks later, the transfection efficiency of AAV viruses was observed by fluorescence microscopy , and the knockdown efficiency was determined by real⁃time PCR of EP3 receptor mRNA on the LPB. The effects of microinjection of saline or PGE2 in the LPB or intraperitoneal injection of LPS on body temperature (Tcore ) and energy expenditure (EE) of shRNA⁃control group and shRNA⁃EP3 group were monitored using an animal monitoring system with temperature telemetry.
Results : AAV virus transfecnificant difference in basal body temperature between shRNA⁃control group and shRNA⁃EP3 group. Tcore and EE were briefly and slightly increased after microinjection of saline in the LPB , but there was no significant difference between the two groups. Compared with the shRNA⁃control group , the febrile response induced by LPB PGE2 was attenuated in the shRNA⁃EP3 group (P < 0. 05) . Furthermore , the knockdown of EP3 receptor of LPB also attenuated the LPS⁃induced fever, and the Tcore 5. 5 h post⁃LPS in the shRNA⁃EP3 rats increased compared with the baseline (P < 0. 05) , which was lower than that in the shRNA⁃control rats ( P < 0. 01) .
Conclusion :EP3 receptor knockdown in LPB attenuates the febrile response induced by microinjection of PGE2 in the LPB and intraperitoneal injection of LPS , suggesting that EP3 receptors of LPB mediate the pyrogenic action of LPB PGE2 and partly participate in LPS⁃induced fever.
- Full text:2024071017095349512腺相关病毒介导的shRNA...EP3受体对发热反应的影响_何田慧.pdf
