The construction of a prognostic model for colorectal cancer based on immune gene correlation and drug screening
10.19405/j.cnki.issn1000-1492.2024.05.007
- VernacularTitle:基于免疫基因相关的结直肠癌预后模型的构建分析及药物筛选
- Author:
Wei ZHENG
1
;
Jiajia ZHAO
;
Xiang CHENG
;
Hongxin TAN
;
Qi HUANG
Author Information
1. 安徽医科大学第二附属医院肿瘤科,合肥 230601
- Keywords:
colorectal cancer;
immune checkpoint inhibitors;
tumour microenvironment;
prognostic models;
immune-related genes
- From:
Acta Universitatis Medicinalis Anhui
2024;59(5):789-796
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search for new biomarkers to predict prognosis in colorectal cancer (CRC) patients.Methods A prognostic model was developed for colorectal cancer with immune-related genes from the cancer ge-nome atlas (TCGA) database using one-way Cox regression analysis and least absolute shrinkage and selection op-erator (LASSO) regression analysis.Moreover, the immune infiltration characteristics of patients in high and low risk groups was compared by sstimation of stromal and immune cells in malignant tumor tissues using expression da-ta (ESTIMATE) and cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) .In addition, the expression levels of immune checkpoints were analyzed in patients from different risk groups.The sen-sitivity of patients in the two risk groups to chemotherapeutic agents was also compared based on genomics of drug sensitivity in cancer (GDSC).Results It was found that the prognostic model constructed based on immune genes could better predict the overall survival (OS) of CRC patients,and the results showed area under curve (AUC) values of 0.764 (95％ CI:0.751-0.793), 0.773 (95％ CI:0.761 -0.779), and 0.760 (95％ CI:0.742 -0.774) for 1-, 3-, and 5-year OS, respectively.Patients in the low-risk group had higher expression levels of im-mune checkpoints and more abundant immune cells such as T cells (P<0.001) , dendritic cells (P<0.001) , macrophages (P<0.001) , neutrophils (P<0.001) .Patients in the high-risk group might be more sensitive to some chemotherapeutic agents such as axitinib, imatinib, methotrexate, pazopanib, rapamycin, sunitinib and tasig-arnib.Conclusion A prognostic model based on 19 immune genes was effective in predicting the prognosis of CRC patients.The number and activity of immune cells in the immune microenvironment in different patients may be an important factor influencing their response to immunocheck inhibitors and chemotherapeutic agents.
- Full text:2024071010215094196基于免疫基因相关的结直肠癌...后模型的构建分析及药物筛选_郑伟 (1).pdf
