Optimization and methods of culture in vitro of astrocytes from cerebral cortical mice
10.19405/j.cnki.issn1000-1492.2024.05.005
- Author:
Nana Xue
1
;
Caiqi Xu
1
;
Yongrong Shi
1
;
Rui Zhang
1
;
Qian Meng
1
Author Information
1. Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Antiinflammatory and Immune Medicine ,Ministry ofEducation , Anhui Collaborative Innovation Center of Anti⁃inflammatory and Immuno Drugs , Hefei 230032
- Publication Type:Journal Article
- Keywords:
astrocyte;
cerebral cortex;
primary culture;
cell purification;
glial fibrillary acidic protein
- From:
Acta Universitatis Medicinalis Anhui
2024;59(5):774-779
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore and optimize the in vitro primary culture method of astrocytes in neonatal mouse
cerebral cortex , which provides a better solution for the in vitro culture of astrocytes.
Methods :In order to optimize the in vitro culture method of mouse cerebral cortex astrocytes , 3 ⁃day⁃old C57BL/6J mouse cerebral cortex tissues were taken , meninges and blood vessels were removed , digested by pancreatic enzymes and centrifuged , andhigh⁃glucose dulbecco ′s modified eagle medium (DMEM) was added to form cell suspension , which was purified by differential adhesion method , cross hand method and constant temperature shaking method.The cells were inoculated in poly⁃D ⁃lysine⁃coated culture bottles with different culture densities , and the purity of astrocytes was determined by morphological ob servation and immunofluorescence staining.
Results :The cells were inoculated at a density of 5 × 106 cells per bottle with good effect and high activity. The purity of astrocytes reached 99% by using high sugar DMEM medium combined with differential adhesion method , cross hand method and constant temperature shaking method.
Conclusion :The primary culture method of astrocytes in mouse cerebral cortex is successfully established and optimized.
- Full text:2024071009550527753小鼠大脑皮质星形胶质细胞的体外培养方法及优化_薛娜娜.pdf
