The effect and mechanism of PPP5C on the migration and invasion and tumor stemness of human lung adenocarcinoma H1299 cells
10.19405/j.cnki.issn1000-1492.2023.01.016
- Author:
Lili Zeng
1
;
Weiwei Chen
2
;
Xiangrui Ma
3
;
Hongliang Dong
2
;
Jing Du
2
Author Information
1. Medical Research Center, Dept of Oral and Maxillofacial Surgery, Binzhou Medical University Hospital , Binzhou 256600
2. Medical Research Center,Binzhou Medical University Hospital , Binzhou 256600
3. Dept of Oral and Maxillofacial Surgery, Binzhou Medical University Hospital , Binzhou 256600
- Publication Type:Journal Article
- Keywords:
PPP5C;
cell proliferation;
migration and invasion;
tumor stemness;
MMP9;
SOX2
- From:
Acta Universitatis Medicinalis Anhui
2023;58(1):94-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of phosphoprotein phosphatase 5 catalytic(PPP5C) on the migration , invasion and tumor stemness of human lung adenocarcinoma H1299 cells.
Methods:The PPP5C⁃pcDNA3. 1 overexpression vector was constructed. PPP5C⁃pcDNA3. 1 and pcDNA3. 1 were transfected into H1299 cells , and H1299 stable cell lines were screened with G418. The mRNA and protein expression levels of PPP5C were identified by qRT⁃PCR and Western blot. The proliferation activity of H1299 cells was detected by drawing cell growth curve and cell clonal formation assay. The wound⁃healing assay and transwell assay were used to test the migration and invasion abilities of H1299 cells , respectively. The stemness of H1299 cells was evaluated by sphere formation assay.
Results:The PPP5C⁃pcDNA3. 1 eukaryotic expression vector was successfully constructed and the expression levels of PPP5C significantly increased after transfection into H1299 cells. After overexpression of PPP5C in H1299 cells , the cell growth curve and clonal formation assay displayed that the proliferation ability was not affected , the migration and invasion of cells were significantly enhanced through wound⁃healing assay and transwell assay , accompanied by an increase in the expression of MMP9 , stem cell spheroid assay showed a significant increase in stemness of cells , accompanied by increased expression of SOX2.
Conclusion:The proliferation ability of cells is not affected , the migration and invasion and the stemness of cells are enhanced by regulating MMP9 and SOX2 respectively , after overexpression of PPP5C in human lung adenocarcinoma H1299 cells.
- Full text:202407071629162113PPP5C对人肺腺癌H12...及肿瘤干性的影响及机制研究_曾莉莉.pdf
