The effects of sulforaphane on proliferation , migration and invasion of human renal carcinoma cells and its mechanism
10.19405/j.cnki.issn1000-1492.2023.01.014
- Author:
Yuxiang Zhao
1
;
Wangyu Wu
1
;
Zhouting Tuo
1
;
Weiwei Qian
1
;
Dexin Yu
1
;
Tao Zhang
1
Author Information
1. Dept of Urology, The Second Afiliated Hospital of Ahui Medical University, Hefei 230601
- Publication Type:Journal Article
- Keywords:
sulforaphane;
kidney cancer cells;
EMT;
nuclear factor kappa B predominate
- From:
Acta Universitatis Medicinalis Anhui
2023;58(1):79-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of sulforaphane (SFN) on proliferation , migration and invasion of human renal carcinoma cells and its mechanism.
Methods:The cultured human renal carcinoma cells 786⁃O were divided into control group (0 μmol/L) and SFN group (5 , 10 , 20 μmol/L) . The activated proliferation of cells was detected by CCK⁃8 ; the effect of SFN on migration of 786⁃O cells was detected by scratch healing assay and Transwell cell migration assay; the effect of SFN on the invasion ability of 786⁃O cells was detected by Transwell cell invasion ability assay; Western blot and qRT⁃PCR were used to detect the effects of SFN on the expression of epithelial⁃mesenchymal transition (EMT) Ⅳrelated proteins and mRNA. The effect of SFN on the expression of NF⁃κB signaling pathway was detected by Western blot.
Results:After SFN treatment for 24 , 48 and 72 h , the proliferation activity of 786⁃O cells decreased with the increase of SFN concentration ; compared with the control group , the cell migration and invasion ability of SFN⁃treated group were significantly reduced ; with the increase of SFN concentration , the mRNA and protein expression levels of E ⁃cadherin in 786⁃O cells increased , while the mRNA and protein expression levels of N ⁃cadherin and Vimentin decreased ; the levels of NF⁃κB signaling pathway related protein phosphorylated p65 and phosphorylated IκBα decreased with the increase of SFN concentration.
Conclusion:SFN may inhibit the proliferation , migration and invasion of human renal carcinoma cells by regulating the EMT process of renal carcinoma through inhibition of NF⁃κB signaling pathway.
- Full text:2024070710464174247莱菔硫烷对人肾癌细胞增殖、迁移和侵袭的影响及作用机制_赵余祥.pdf
