Study on the neuroprotective mechanism of mesenchymal stem cells combined with low⁃intensity transcranial ultrasound therapy in TBI rats
10.19405/j.cnki.issn1000-1492.2023.01.013
- Author:
Xinyu Yao
1
,
2
,
3
;
Yue Li
4
;
Yansheng Chen
5
;
Juan Du
5
;
Xin Liang
5
;
Lanxiang Liu
5
;
Zhendong Cao
6
Author Information
1. Graduate School of Chengde Medical University, Chengde 067000
2. Dept ofMagnetic Resonance Imaging , The First Hospital of Qinhuangdao , Qinhuangdao 066000
3. Dept ofMagnetic Resonance Imaging , Afiliated Hospital of Chengde Medical University, Chengde 067000
4. Dept of Orthopedics , Qinhuangdao First Hospital , Qinhuangdao 066000
5. Dept ofMagnetic Resonance Imaging , The First Hospital of Qinhuangdao , Qinhuangdao 066000
6. Dept ofMagnetic Resonance Imaging , Afiliated Hospital of Chengde Medical University, Chengde 067000
- Publication Type:Journal Article
- Keywords:
traumatic brain injury;
low⁃intensity transcranial ultrasound;
mesenchymal stem cells;
combined therapy;
neurological impairment
- From:
Acta Universitatis Medicinalis Anhui
2023;58(1):73-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the neuroprotective effect of mesenchymal stem cells (MSC) combined with low⁃intensity transcranial ultrasound (LITUS) treatment on traumatic brain injury (TBI) .
Methods:Seventy⁃two SD rats were randomly divided into four groups , namely , control group , TBI group , MSC injection group , and combined treatment group , with 18 rats in each group. TBI model was established by applying a pneumatic controlled cortical impingement instrument. Within 24 h after surgery , MSC was injected into the injury site by microinjector and microinjector pump using in situ injection. After injection , the injury site was treated with LITUS for 28 consecutive days using an ultrasound stimulator. The modified neurological functioning score ( mNSS) was performed on rats in each group at 1 , 3 , 7 , 14 , 21 and 28 days postoperatively , and then the brains were extracted to detect pathological changes at the injury site and the mRNA and protein expression of brain⁃derived neurotrophic factor (BDNF) , growth associated protein⁃43 ( GAP⁃43) , postsynaptic density protein⁃95 ( PSD⁃95 ) and glial fibrillary acidic protein(GFAP) by HE staining , immunohistochemistry , Western blot and RT⁃PCR.
Results:Compared with the control group , the mNSS score increased in the TBI group (P < 0. 05) , the expression of GAP⁃43 and PSD⁃95 decreased , and the expression of GFAP increased ( P < 0. 05 ) ; Compared with the TBI group , the mNSS score of MSC group was lower (P < 0. 05) , the expression of BDNF , GAP⁃43 , PSD⁃95 increased , and the expression of GFAP decreased (P < 0. 05) ; mNSS scores were lower in the combined treatment group than those in the MSC group (P < 0. 05) , the expression of BDNF , GAP⁃43 , PSD⁃95 increased , and the expression of GFAP decreased (P < 0. 05) .
Conclusion:The mechanism by which MSC combined with LITUS exerts neuroprotective effects in TBI may be related to the promotion of BDNF , GAP⁃43 , and PSD⁃95 expression and reduction of GFAP expression.
- Full text:2024070710160991538间充质干细胞联合低强度经颅...大鼠的神经保护作用机制研究_姚欣雨.pdf
