The effects of hypoxia on silencing HLA⁃G expression and biological behaviors of JEG⁃3 cells and EPAS1
10.19405/j.cnki.issn1000-1492.2023.01.009
- Author:
Chunlei Sun
1
,
2
;
Juan Yang
3
;
Yingying Xie
1
,
2
;
Wenyu Xu
1
,
2
;
Juan Lei
1
,
2
;
Duoduo Liu
1
,
2
Author Information
1. Graduate School of Qinghai University, Xining 810000
2. Dept of Obstetrics , Afiliated Hospital of Qinghai University, Xining 810000
3. Dept of Obstetrics , Afiliated Hospital of Qinghai University, Xining 810000
- Publication Type:Journal Article
- Keywords:
preeclampsia;
hypoxia;
human leukocyte antigen⁃G;
endothelial PAS1 region protein 1;
high altitude
- From:
Acta Universitatis Medicinalis Anhui
2023;58(1):47-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of silencing human leukocyte⁃associated antigen⁃G ( HLA⁃G) expression in the chorionic trophoblast cell line JEG⁃3 cells under hypoxic conditions on the biological function of JEG⁃3 cells and through the hypoxia response pathway of endothelial PAS1 region protein 1 (EPAS1)is involved in the molecular mechanism of preeclampsia under high altitude hypoxia.
Methods:The expression of HLA⁃G in JEG⁃3 cells was inhibited by transfection of small interfering RNA (siRNA) . The JEG⁃3 cells after transfection were divided into four groups : normoxic control group , hypoxic control group , normoxic inhibition group , and hypoxic inhibition group. CCK⁃8 test and Transwell test were used to detect the proliferation and invasion ability of the cells in four groups ; The effects of four groups of apoptosis and cell cycle were detected by flow cytometry; HLA⁃G and EPAS1 mRNA and protein expression levels were detected by real⁃time fluorescence quantitative PCR ( qPCR) and Western blot.
Results :① Compared with the normoxic control group , hypoxic control group , normoxic inhibition group , and hypoxic inhibition group could reduce the proliferation activity and invasion ability of JEG⁃3 cells , and significantly increase the apoptosis rate. The hypoxic control group and hypoxic inhibition group also produced anobvious necrotic cell population ; Under the condition of hypoxia , after reducing the expression of HLA⁃G , the cell necrosis rate was further aggravated ; Whether under normoxia or hypoxia , inhibition of HLA⁃G expression caused the cells to be blocked in the G1 phase. ② Compared with the normoxic control group , hypoxia control group , normoxic inhibition group , and hypoxia inhibition group decreased the expression of HLA⁃G protein , and hypoxia and inhibition of HLA⁃G had a synergistic effect; Hypoxia⁃inducible factors⁃2α ( HIF⁃2α ) , vascular endothelial growth factor(VEGF) and endothelin⁃1(ET⁃1) protein expression could be added , inhibition of HLA⁃G decreased the expression of inducible nitric oxide synthase( NOS2) .
Conclusion:In the hypoxic environment , silencing HLA⁃G may affect the biological behavior of trophoblast through the EPAS1 hypoxic response pathway and participate in the
occurrence and development of preeclampsia.
- Full text:2024070518424327338低氧下沉默HLA-G对JE...1表达及其生物学行为的影响_孙春蕾.pdf
