Effects of aflatoxin B1 on the biophysical properties and cytoskeleton structure of hepatocellular carcinoma cell line HepG2
10.19405/j.cnki.issn1000-1492.2023.01.003
- Author:
Huan Yu
1
;
Yanqin Yu
2
;
Tianbao Qian
1
;
Qingyu Liu
1
;
Yun Wang
3
;
Zhu Zeng
3
;
Zuquan Hu
4
Author Information
1. Immune Cells and Antibody Engineering Research Center in University of Guizhou Province , School ofBiology and Engineering
2. Immune Cells and Antibody Engineering Research Center in University of Guizhou Province , School ofBiology and Engineering,Key Laboratory ofInfectious Immune and Antibody Engineering of Guizhou Province , Engineering Research Center for Cellular Immunotherapy of Guizhou Province , School of Basic Medical Sciences , Guizhou Medical University, Guiyang 550025
3. Key Laboratory ofInfectious Immune and Antibody Engineering of Guizhou Province , Engineering Research Center for Cellular Immunotherapy of Guizhou Province , School of Basic Medical Sciences , Guizhou Medical University, Guiyang 550025
4. Immune Cells and Antibody Engineering Research Center in University of Guizhou Province , School ofBiology and Engineering, Key Laboratory ofInfectious Immune and Antibody Engineering of Guizhou Province , Engineering Research Center for Cellular Immunotherapy of Guizhou Province , School of Basic Medical Sciences , Guizhou Medical University, Guiyang 550025
- Publication Type:Journal Article
- Keywords:
aflatoxin;
hepatocellular carcinoma cells;
biophysical properties;
cytoskeleton
- From:
Acta Universitatis Medicinalis Anhui
2023;58(1):10-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of aflatoxin B1 (AFB1) on the biophysical properties and cytoskeleton structure of human hepatocellular carcinoma cells (HCCs) .
Methods:HepG2 cells were respectively treated with 0 , 0. 01 , 0. 1 , 1 , 5 , 10 μmol/L AFB1 for 24 h and 48 h , and the cell viability was measured by CCK⁃8 kit.Based on this result , the influences of 10 μmol/L AFB1 on the osmotic fragility , membrane fluidity , electrophoretic mobility (EPM) and F ⁃actin structure of cells were analyzed. Subsequently , total RNAs were extracted and the PCR.
Results:The increased viability of HepG2 cells was induced by AFB1 in a dose⁃dependent manner after 48h
treatment. After treated with 10 μmol/L AFB1 , the anti⁃hypotonic ability and EPM of HepG2 cells were en⁃hanced. The content of F ⁃actin in HepG2 cells increased obviously , while the mRNA expression levels of the main cytoskeleton binding proteins were altered.
Conclusion :AFB1 can affect the biophysical properties , cytoskeleton structure and its binding proteins of HepG2 cells , which may be directly related to its toxic action.
- Full text:2024070321144866840黄曲霉毒素B1对肝癌细胞H...学特性及细胞骨架结构的影响_于欢.pdf
