Golgi protein GOLPH3 regulates lysosomal biogenesis through mTORC1 signaling
10.19405/j.cnki.issn1000-1492.2023.01.00
- Author:
Yuan Sun
1
;
Rong Qiu
1
;
Xinwang Cao
1
Author Information
1. Dept of Cellular Biology, School ofLife Sciences , Anhui Medical University,Hefei 230032
- Publication Type:Journal Article
- Keywords:
GOLPH3;
mTORC1;
Golgi apparatus;
lysosome biogenesis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the mechanism of Golgi phosphoprotein 3 (GOLPH3) regulating lysosomal biogenesis via mTORC1 signaling.
Methods :GOLPH3 knockout (GOLPH3 KO) stable cell line was constructed by CRISPR Cas9. mTORC1 activity and the levels of TFEB and p ⁃TFEB in the control cells and GOLPH3 KO cells were compared through Western blot. Further, lysosome⁃associated membrane protein LAMP1 was labelled by the means of immunofluorescence and the number of lysosomes in the control cells and GOLPH3 KO cells was com⁃pared.
Results : GOLPH3 KO suppressed mTORC1 activity significantly , decreased the cytoplasm level of p ⁃TFEB , increased the nuclear localization of active TFEB , and promoted the lysosome biogenesis.
Conclusion:Golgi protein GOLPH3 regulates lysosome biogenesis through mTORC1 signaling.
- Full text:2024070320383404871高尔基体蛋白GOLPH3通...路调控溶酶体发生的分子机制_孙源.pdf
