Mechanism of puerarin targeting the mitochondria to inhibit proliferation and invasion of breast cancer cells
10.19405/j.cnki.issn1000-1492.2024.04.011
- VernacularTitle:葛根素靶向线粒体抑制乳腺癌细胞增殖和侵袭的机制
- Author:
Caihan YU
1
,
2
;
Dai LI
;
Min XIE
Author Information
1. 湖北科技学院医学部药学院,咸宁 437100
2. 咸宁市中心医院(湖北科技学院第一附属医院)眼科,咸宁 437100
- Keywords:
puerarin;
mitochondria;
breast cancer;
network pharmacology;
molecular docking;
DST
- From:
Acta Universitatis Medicinalis Anhui
2024;59(4):627-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of puerarin on the proliferation,invasion and apop-tosis of breast cancer cell HCC1806 via network pharmacology,molecular docking and in vitro experiments.Meth-ods The data of breast cancer and mitochondrial diseases were collected from GEO database,and the differentially expressed genes were analyzed by GEO2R.Overlapping genes between breast cancer and mitochondrial database were analyzed by Venn diagram.GO and KEGG enrichment analyzed the overlapping genes.STRING and Cyto-scape analyzed overlapping gene interaction networks and screen core genes.Interaction between puerarin and core genes was docked with autodock.Cell proliferation,apoptosis and invasion capacity were measured by CCK-8,EdU,TUNEL and Transwell experiments,mitochondrial membrane potential was measured by Mito-Tracker experi-ments and protein expression levels were measured by Western blot.Anti-tumor efficacy of puerarin was analyzed by subcutaneous xenograft of breast cancer and immunohistochemical assay in vivo.Results Among 132 overlap-ping genes in breast cancer and mitochondrial disease,10 core differentially expressed genes were selected.Among these core genes,dual serine/threonine and tyrosine protein kinase(DST)was low expressed in breast cancer tis-sues.Puerarin bound to DST,up-regulated its expression,inhibited the proliferation and invasion of HCC1806 breast cancer cells,promoted breast cell apoptosis,increased the expression levels of apoptosis-related proteins Cleaved-Caspase 3 and Bax,down-regulated the expression of anti-apoptosis protein Bcl-2,and decreased mito-chondrial membrane potential.In vivo,puerarin inhibited the growth of breast cancer and up-regulated the expres-sion of DST in tumor tissues.Conclusion Puerarin inhibits the proliferation and invasion of breast cancer cells,promotes the apoptosis of cancer cells and inhibits breast cancer growth.
