Mechanism of puerarin targeting the mitochondria to inhibit proliferation and invasion of breast cancer cells
10.19405/j.cnki.issn1000-1492.2024.04.011
- Author:
Caihan Yu
1
,
2
;
Dai Li
1
;
Min Xie
1
Author Information
1. School of Pharmacy , Xianning Medical College , Hubei University of Science and Technology , Xianning 437100
2. Dept of Ophthalmology , Xianning Central Hospital , The First Afiliated Hospital of Hubei University of Science and Technology , Xianning 437100
- Publication Type:Journal Article
- Keywords:
puerarin;
mitochondria;
breast cancer;
network pharmacology;
molecular docking;
DST
- From:
Acta Universitatis Medicinalis Anhui
2024;59(4):627-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect and mechanism of puerarin on the proliferation , invasion and apop- tosis of breast cancer cell HCC1806 via network pharmacology , molecular docking and in vitro experiments .
Methods:The data of breast cancer and mitochondrial diseases were collected from GEO database , and the differentially expressed genes were analyzed by GEO2R . Overlapping genes between breast cancer and mitochondrial database were analyzed by Venn diagram . GO and KEGG enrichment analyzed the overlapping genes . STRING and Cyto- scape analyzed overlapping gene interaction networks and screen core genes . Interaction between puerarin and core genes was docked with autodock . Cell proliferation , apoptosis and invasion capacity were measured by CCK-8 , EdU , TUNEL and Transwell experiments , mitochondrial membrane potential was measured by Mito-Tracker experi- ments and protein expression levels were measured by Western blot. Anti-tumor efficacy of puerarin was analyzed by subcutaneous xenograft of breast cancer and immunohistochemical assay in vivo .
Results :Among 132 overlap- ping genes in breast cancer and mitochondrial disease , 10 core differentially expressed genes were selected . Among these core genes , dual serine/threonine and tyrosine protein kinase (DST) was low expressed in breast cancer tis- sues . Puerarin bound to DST , up-regulated its expression , inhibited the proliferation and invasion of HCC1806 breast cancer cells , promoted breast cell apoptosis , increased the expression levels of apoptosis-related proteins Cleaved-Caspase 3 and Bax , down-regulated the expression of anti-apoptosis protein Bcl-2 , and decreased mito- chondrial membrane potential . In vivo , puerarin inhibited the growth of breast cancer and up-regulated the expres- sion of DST in tumor tissues .
Conclusion :Puerarin inhibits the proliferation and invasion of breast cancer cells , promotes the apoptosis of cancer cells and inhibits breast cancer growth .
- Full text:2024070319595223519葛根素靶向线粒体抑制乳腺癌细胞增殖和侵袭的机制_余才翰.pdf
