Phenotype of peroxisome proliferator-activated receptor-alpha(PPARalpha)deficient mice on mixed background fed high fat diet.
- Author:
Bang Hyun KIM
1
;
Young Suk WON
;
Eun Young KIM
;
Mijung YOON
;
Ki Taek NAM
;
Goo Taeg OH
;
Dae Yong KIM
Author Information
1. Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration, Seoul 122-704, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Phenotype;
PPAR alpha-/- mice;
C57BL/6Nx129/Sv;
high fat diet
- MeSH:
Adipose Tissue/metabolism;
Animals;
Body Weight;
Cholesterol/blood;
Crosses, Genetic;
Dietary Fats/*administration & dosage;
Female;
Histocytochemistry;
Liver/enzymology/metabolism;
Male;
Mice;
Mice, Inbred C57BL;
Mice, Knockout;
Obesity/genetics/*metabolism;
Phenotype;
RNA/chemistry/genetics;
Receptors, Cytoplasmic and Nuclear/*deficiency/genetics/metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Specific Pathogen-Free Organisms;
Transcription Factors/*deficiency/genetics/metabolism;
Triglycerides/blood
- From:Journal of Veterinary Science
2003;4(3):239-244
- CountryRepublic of Korea
- Language:English
-
Abstract:
Considerable controversy exists in determining the role of peroxisome proliferator-activated receptor-alpha(PPARalpha) on obesity. Previous reports demonstrated that PPARalpha is a critical modulator of lipid homeostasis, but the overt, obese phenotypic characterization in the strain of PPAR deficient (PPARalpha-/-) mice is influenced by other factors, including diet and genetics. Therefore, it is necessary to establish the phenotypic characterization of PPARalpha-/- mice prior to the obesity-related study. In this study, we observed phenotype of PPARalpha-/- mice on mixed genetic background (C57BL/6Nx129/Sv) fed a high fat diet for 16 weeks. PPARalpha-/- mice, regardless of sex, raised body growth rate significantly comparing with wild type and showed male-specific fatty change in the liver. They were shown to lack hepatic induction of PPARalpha target genes encoding enzymes for fatty acid beta-oxidation.
