Screening and identification of core genes for diabetic nephropathy
10.19405/j.cnki.issn1000-1492.2024.04.009
- VernacularTitle:糖尿病肾病核心基因的筛选与鉴定
- Author:
Xiaojun WU
1
,
2
;
Feixue NI
;
Yuxue XU
Author Information
1. 滨州医学院药学院,烟台 264003
2. 滨州医学院山东省分子靶向智能诊疗技术创新中心,烟台 264003
- Keywords:
diabetic kidney disease;
GEO database;
transcriptome;
bioinformatics;
genetic screening;
drug target
- From:
Acta Universitatis Medicinalis Anhui
2024;59(4):610-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the core genes of diabetic kidney disease(DKD)based on bioinformatics,ex-plore the therapeutic targets of DKD,and discuss its possible regulatory mechanism.Methods The expression da-ta matrix of glomerular transcriptome in patients with DKD in GEO database(GSE30528,GSE47183)was extrac-ted,and the differentially expressed genes(DEGs)were screened by bioinformatics methods to identify the core differential genes,and then gene expression and enrichment analysis(GSEA)were conducted to predict effective targets.Results By screening and identifying the mRNA expression matrix of DKD,five core genes were screened out.Among them,C1orf21 and NPHS1 were significantly down regulated,and CD48,COL1A2,and TGFBI were up regulated.NPHS1 and CD48 were significantly related to immune differences,intercellular communication,and cell surface interaction.Through receiver operating characteristic curve(ROC)analysis and GSEA analysis and drug target prediction,it might be of great significance for the treatment of DKD.Conclusion The core genes screened in this study have significant correlation with DKD,which may be used as effective markers for the treat-ment of diabetes.And then,this study provides a theoretical basis for the treatment and identification of DKD.
