Screening and identification of core genes for diabetic nephropathy
10.19405/j.cnki.issn1000-1492.2024.04.009
- Author:
Xiaojun Wu
1
,
2
;
Feixue Ni
1
,
2
;
Yuxue Xu
1
,
2
,
3
Author Information
1. School of Pharmacology , Binzhou Medical University , Yantai 264003
2. Shandong Technology Innovation Center of Molecular Targeting and Intelligent Diagnosis and Treatment , Binzhou Medical University , Yantai 264003
3. Yantai Stem Cell and Regenerative Medicine Key Laboratory , Binzhou Medical University , Yantai 264003
- Publication Type:Journal Article
- Keywords:
diabetic kidney disease;
GEO database;
transcriptome;
bioinformatics;
genetic screening;
drug target
- From:
Acta Universitatis Medicinalis Anhui
2024;59(4):610-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To screen the core genes of diabetic kidney disease ( DKD) based on bioinformatics , ex- plore the therapeutic targets of DKD , and discuss its possible regulatory mechanism .
Methods :The expression da- ta matrix of glomerular transcriptome in patients with DKD in GEO database (GSE30528 , GSE47183) was extrac- ted , and the differentially expressed genes ( DEGs) were screened by bioinformatics methods to identify the core differential genes , and then gene expression and enrichment analysis (GSEA) were conducted to predict effective targets .
Results :By screening and identifying the mRNA expression matrix of DKD , five core genes were screened out. Among them , C1orf21 and NPHS1 were significantly down regulated , and CD48 , COL1A2 , and TGFBI were up regulated . NPHS1 and CD48 were significantly related to immune differences , intercellular communication , and cell surface interaction . Through receiver operating characteristic curve ( ROC) analysis and GSEA analysis and drug target prediction , it might be of great significance for the treatment of DKD .
Conclusion :The core genes screened in this study have significant correlation with DKD , which may be used as effective markers for the treat- ment of diabetes . And then , this study provides a theoretical basis for the treatment and identification of DKD .
- Full text:202407022220472707糖尿病肾病核心基因的筛选与鉴定_吴晓俊.pdf
