Rapamycin upregulates autophagy inhibits cell proliferation in human umbilical vein endothelial cells
10.19405/j.cnki.issn1000-1492.2024.04.008
- Author:
Yawen Wang
1
;
Yanan Cheng
1
;
Bin Yang
1
;
Bihao Su
1
;
Pu Xu
1
Author Information
1. Dept of Stomatology , Haikou Hospital , Hainan Stomatological Center , Central South University Xiangya School of Medicine Afiliated Haikou Hospital , Haikou 570208
- Publication Type:Journal Article
- Keywords:
rapamycin;
HUVECs;
autophagy;
autophagic activity;
autophagic protein;
cell proliferation
- From:
Acta Universitatis Medicinalis Anhui
2024;59(4):605-610
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of autophagy activation on cell proliferation in human umbilical vein endothelial cells (HUVECs) .
Methods :HUVECs were treated with rapamycin (Rapa) . Western blot assay was performed to examine the expression of protein of microtubule associated protein 1 light chain 3 (LC3) , Beclin 1 and unc-51-like kinase 1 (ULK1) . Autophagosomes were detected by transmission electron microscopy ( TEM) , and autophagy fluorescence was detected by monodansylcadaverine staining(MDC) assay . The effect of autophagy activation on cell proliferation was assessed by CCK-8 assay and EdU assay . Vascular formation experiments were used to detect vasculogenic ability .
Results :After Rapa treatment , LC3 , Beclin1 and ULK1 expressions were en- hanced , while the green autophagy fluorescence expression in the experimental group was stronger than that in the control group , and autophagosomes were visible by TEM ; CCK-8 and EdU results showed that compared with the control group , the cell proliferation ability was weakened and tubes formation ability was reduced after the activation of autophagy in experimental cells .
Conclusion :Rapa upregulates autophagy activity in HUVECs to inhibit cell proliferation under certain time .
- Full text:2024070222160706620雷帕霉素上调人脐静脉内皮细胞自噬活性抑制细胞增殖_王雅雯.pdf
