The lncSIL molecule exerts a negative regulatory effect on the alveolar epithelial-mesenchymal transition induced by TGF-β1 through modulation of the EZH2/P21/CDK6 signaling pathway
10.19405/j.cnki.issn1000-1492.2024.04.007
- VernacularTitle:lncSIL通过EZH2/P21/CDK6信号通路负向调控TGF-β1诱导的肺泡上皮细胞间质转化
- Author:
Wanfang ZHANG
1
;
Lin WANG
;
Pengtao PAN
;
Wenxin LI
;
Ruili KANG
;
Ziren ZHU
;
Haoqin CHEN
;
Xinyu FANG
;
Xingcan ZHANG
;
Yuxin ZHANG
;
Yiwen JIANG
;
Xinyan LI
;
Benqi YUAN
Author Information
1. 新乡学院生物工程学院生物技术教研室,新乡 453003
- Keywords:
lncSIL;
long noncoding RNA;
idiopathic pulmonary fibrosis;
epithelial-mesenchymal transition;
transforming growth factor-β1;
enhancer of zeste homolog 2;
cell marker proteins
- From:
Acta Universitatis Medicinalis Anhui
2024;59(4):600-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of lncSIL in transforming growth factor-β1(TGF-β1)-induced alveo-lar epithelial interstitial transformation(EMT)and its related signaling pathways.Methods Western blot was used to detect the effect of lncSIL silencing on the expression of E-cadherin(E-cad),alpha-smooth muscle actin(α-SMA)and Collagen I(Col I)in the process of EMT induced by TGF-β1.LncSIL interacting proteins were ana-lyzed by RNA pulldown.Western blot was used to detect the effect of overexpression or silencing of lncSIL on the expression of its target gene enhancer of zeste homolog 2(EZH2)and its downstream factors P21 and cyclin-de-pendent kinase 6(CDK6).Flow cytometry was used to analyze the effect of lncSIL on cell cycle progression.Re-sults After lncSIL silencing,the expression of α-SMA and Col I increased,the expression of E-cad decreased.RNA pulldown assay showed that EZH2 was the target protein that interacted with lncSIL,and the expression of EZH2 increased after silencing lncSIL,the expression of EZH2 downstream gene P21 decreased,CDK6 increased.Flow cytometry showed that the number of cells in S phase significantly increased.When lncSIL was overexpressed,the expression of EZH2 and CDK6 was down-regulated,the expression of P21 was up-regulated,and the number of S phase cells significantly decreased.Conclusion LncSIL inhibits TGF-β1-induced alveolar epithelial cell mesen-chymal transition by negatively regulating EZH2/P21/CDK6 signaling pathway to inhibit cell cycle progression.
