The lncSIL molecule exerts a negative regulatory effect on the alveolar epithelial-mesenchymal transition induced by TGF-β1 through modulation of the EZH2/P21/CDK6 signaling pathway

Wanfang Zhang; Lin Wang; Pengtao Pan; Wenxin Li; Ruili Kang; Ziren Zhu; Haoqin Chen; Xinyu Fang; Xingcan Zhang; Yuxin Zhang; Yiwen Jiang; Xinyan Li; Benqi Yuan

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The lncSIL molecule exerts a negative regulatory effect on the alveolar epithelial-mesenchymal transition induced by TGF-β1 through modulation of the EZH2/P21/CDK6 signaling pathway

Author: Wanfang Zhang ¹ ; Lin Wang ² ; Pengtao Pan ³ ; Wenxin Li ² ; Ruili Kang ¹ ; Ziren Zhu ¹ ; Haoqin Chen ² ; Xinyu Fang ² ; Xingcan Zhang ² ; Yuxin Zhang ² ; Yiwen Jiang ² ; Xinyan Li ² ; Benqi Yuan ²
Author Information

1. School of Biological Engineering , Xinxiang University , Xinxiang 453003
2. School of International Education , Xinxiang University , Xinxiang 453003
3. Medical College of Xinxiang University , Xinxiang 453003
Publication Type:Journal Article
Keywords: lncSIL; long noncoding RNA; idiopathic pulmonary fibrosis; epithelial-mesenchymal transition; transforming growth factor-β1; enhancer of zeste homolog 2; cell marker proteins
From: Acta Universitatis Medicinalis Anhui 2024;59(4):600-604
CountryChina
Language:Chinese
Abstract: Objective :To investigate the role of lncSIL in transforming growth factor-β1(TGF-β1)-induced alveo- lar epithelial interstitial transformation (EMT) and its related signaling pathways .
Methods :Western blot was used to detect the effect of lncSIL silencing on the expression of E-cadherin ( E-cad) , alpha-smooth muscle actin ( α- SMA) and Collagen I (Col I) in the process of EMT induced by TGF-β1 . LncSIL interacting proteins were ana- lyzed by RNA pulldown . Western blot was used to detect the effect of overexpression or silencing of lncSIL on the expression of its target gene enhancer of zeste homolog 2 (EZH2) and its downstream factors P21 and cyclin-de- pendent kinase 6 (CDK6) . Flow cytometry was used to analyze the effect of lncSIL on cell cycle progression .
Results:After lncSIL silencing , the expression of α-SMA and Col I increased , the expression of E-cad decreased . RNA pulldown assay showed that EZH2 was the target protein that interacted with lncSIL , and the expression of EZH2 increased after silencing lncSIL , the expression of EZH2 downstream gene P21 decreased , CDK6 increased . Flow cytometry showed that the number of cells in S phase significantly increased . When lncSIL was overexpressed , the expression of EZH2 and CDK6 was down-regulated , the expression of P21 was up-regulated , and the number of S phase cells significantly decreased .
Conclusion :LncSIL inhibits TGF-β1-induced alveolar epithelial cell mesen- chymal transition by negatively regulating EZH2/P21 /CDK6 signaling pathway to inhibit cell cycle progression .
Full text:2024070222104481959lncSIL通过EZH2_...诱导的肺泡上皮细胞间质转化_张万方.pdf