Preparation and characterization of folic acid modified D ⁃α⁃tocopheryl polyethylene glycol 1000 succinate nanomaterials encapsulated with siRNA
10.19405/j.cnki.issn1000-1492.2023.11.010
- Author:
Manman Zhu
1
;
Yong Cheng
1
;
Peng Rao
1
;
Guiyang Zhang
2
;
Hao Liu
3
;
Lei Xiao
4
;
Jiatao Liu
1
,
5
Author Information
1. School of Pharmacy, Anhui Medical University, Hefei 230032
2. School of Basic Medicine , Anhui Medical University, Hefei 230032
3. Dept of Oncology, The First Afiliated Hospital of Anhui Medical University, Hefei 230022
4. Dept of Pharmacy, The First Afiliated Hospital of Anhui Medical University, Hefei 230022
5. Dept ofPharmacy, The First Afiliated Hospital of Anhui Medical University, Hefei 230022
- Publication Type:Journal Article
- Keywords:
folic acid;
D ⁃α ⁃tocopheryl polyethylene glycol 1000 succinate;
siRNA;
macrophages;
X ⁃box binding protein 1
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1859-1864
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To construct folate modified D ⁃α ⁃tocopheryl polyethylene glycol 1000 succinate ( TPGS) oparticles on the cytotoxicity and spliced X ⁃box binding protein 1 ( XBP1s) of mouse leukemia cells of monocyte macrophage (RAW264. 7) .
Methods : Mixed FA⁃TPGS and rhodamine B (RhB) labeled XBP1 siRNA solution in a proportion of 5 ∶ 1 and obtained the nano⁃complex coated with XBP1 siRNA(FT@ XBP1) . FT@ XBP1 nanocarriers were characterized by transmission electron microscope , dynamic light scattering , ultraviolet visible spectrum analysis and/or fluorescence analysis. And the release of siRNA from FA⁃TPGS nano⁃carriers was calculated simultaneously. The cell cytotoxicity of FT@ XBP1 nanomaterials were detected by scanning electron microscopy (SEM) , CCK⁃8 and flow cytometry. And the inhibited effect of XBP1 s of RAW264. 7 cells was checked by Western blot.
Results :FA modified TPGS could effectively bind XBP1 siRNA. And the average particle size of FT@ XBP1 nanocarriers were(200 ± 20) nm. The relative release assays showed that acidic environments (pH 5. 0) was beneficial for siRNA to release from FT@ XBP1 . Both CCK⁃8 and apoptosis assay showed that the effects of FT@ XBP1 on the proliferation and apoptosis of RAW264. 7 cells were relatively small , and FT@ XBP1 could significantly inhibit the expression of XBP1 s protein in RAW264. 7(P < 0. 001) . Conclusion TPGS nanoparticles modified with folic acid can effectively encapsulate XBP1 siRNA and inhibit XBP1 s expression of RAW264. 7 cells with good cellular compatibility.
- Full text:202407021740019001低温下有氧运动干预对糖尿病...病肾脏代谢和短期预后的影响_王琳琳.pdf
