Changes in glial fibrillary acidic protein expression in different brain regions of rats with traumatic brain inj ury

Yan Cao; Hengxi Li; Jiali Li; Tenghan Ling; Aiping Yin; Ying Zhou; Li Yang; Haiying Wu; Ping Li

Return

Changes in glial fibrillary acidic protein expression in different brain regions of rats with traumatic brain inj ury

Author: Yan Cao ^{1
,

2} ; Hengxi Li ^{1
,

2} ; Jiali Li ^{1
,

2} ; Tenghan Ling ^{1
,

2} ; Aiping Yin ^{1
,

2} ; Ying Zhou ³ ; Li Yang ⁴ ; Haiying Wu ⁴ ; Ping Li ^{1
,

2}
Author Information

1. Dept of Human Anatomy and Histology &
2. Embryology, Kunming Medical University, Kunming 650500
3. Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming 650500
4. Dept of Emergency, The First Afiliated Hospital of Kunming Medical University, Kunming 650032
Publication Type:Journal Article
Keywords: traumatic brain injury; astrocyte; GFAP; cortex; midbrain; rats
From: Acta Universitatis Medicinalis Anhui 2023;58(11):1828-1834, 1840
CountryChina
Language:Chinese
Abstract: Objective :To observe the expression of glial fibrillary acidic protein (GFAP) in the brain tissue of rats with traumatic brain injury (TBI) and the differential changes between brain regions.
Methods :The TBI model of rats was prepared by the modified Feeney free fall method , and it was randomly divided into the sham⁃operated qPCR were used to observe the morphological changes of activated astrocytes and the expression of GFAP in different brain regions after TBI.
Results :Immunohistochemical staining showed that astrocytes were activated in all brain regions on the right side of TBI , and the only brain regions that spilled over to the contralateral side ( left) were cortical region 1 and midbrain regions. RT⁃qPCR results showed that GFAP mRNA was highly expressed in all brain regions on the right side of TBI , affecting the contralateral cortex and midbrain regions. Western blot results showed that GFAP protein was highly expressed in all brain regions on the right side of TBI , and it spread to the contralateral midbrain regions.
Conclusion :TBI can cause the activation of astrocytes and increase GFAP expression in the right brain regions , while also affecting the contralateral (left) cortex 1 and midbrain regions.
Full text:2024070216491777975创伤性脑损伤大鼠不同脑区胶质纤维酸性蛋白表达的变化_曹艳.pdf