Preparation and biocompatibility of a bilayer chitosan barrier membrane loaded with tannic acid
10.19405/j.cnki.issn1000-1492.2024.04.001
- Author:
Xiaonian Yang
1
;
Duohong Zou
1
,
2
Author Information
1. College and Hospital of Stomatology , Anhui Medical University , Key Laboratory of Oral Diseases Research of Anhui Province , Hefei 230032
2. Shanghai Ninth People s Hospital , Shanghai JiaoTong University School of Medicine , Shanghai JiaoTong University School of Stomatology , Shanghai Key Laboratory of Stomatology , Shanghai 200011
- Publication Type:Journal Article
- Keywords:
tannic acid;
chitosan;
reactive oxygen species;
antibacterial;
barrier membrane;
directional freezing
- From:
Acta Universitatis Medicinalis Anhui
2024;59(4):563-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the feasibility of the bilayer chitosan barrier membrane loaded with tannic acid (CS@ TA) for guided bone regeneration by exploring the reactive oxygen species (ROS) scavenging ability , bio- compatibility , and antibacterial properties .
Methods :The single-layer chitosan (CS) film was prepared by self-evaporation , and the double-layer CS film was prepared by directional freezing and freeze-drying , and its microstruc- ture was ob served by scanning electron microscope . The prepared CS bilayer membrane was grafted with tannic acid (TA) in different proportions , and the interaction between TA and CS bilayer membrane was analyzed by Fourier infrared spectrometer (FITR) . The ROS scavenging ability was tested by 1 , 1 -diphenyl-2 -picrylhydrazyl (DPPH) , and the double-layer membrane of loading TA scavenging efficiency of more than 90% was selected to continue the follow-up experiment. CCK-8 assay and lived dead staining were used to evaluate the survival rate of cell in each groups . MC3T3 -E1 cells was adhesion the CS@ TA barrier film for studying by SEM . Colony counting was performed to test its antibacterial performance against Escherichia coli ( E. coli) and Staphylococcus aureus ( S. aureus ) .
Results :One side was the smooth , dense while other side was rough , loose and porous , with a longitudinal ordered porous structure in cross-section of the double-layer membrane of CS@ TA . With the addition of TA , the ROS scavenging ability of the double-layer membrane first increased rapidly and then stabilized slowly. The results of CCK-8 and lived and dead cells staining showed that excessive TA addition significantly affected the biocompati- bility of the double-layer membrane . The counting results of bacterial dilution coating showed that compared with the double-layer membrane without TA loading , the double-layer membrane had certain antibacterial ability against E. coli and S. aureus when the appropriate amount of TA was added .
Conclusion :Thus the double-layer with ap- propriate TA loading has strong ROS scavenging ability , good biological performance , and certain antibacterial abil- ity for E. coli and S. aureus .
- Full text:2024062809073384535负载单宁酸的双层壳聚糖屏障...的制备及其生物相容性的研究_杨肖念.pdf
