Changes in renal cell glycolysis and amino acid metabolism during cisplatin-induced acute kidney injury
- VernacularTitle:顺铂致急性肾损伤肾脏糖酵解和氨基酸代谢改变的研究
- Author:
Shen XU
1
;
Nannan LIANG
;
Yahui REN
;
Yizhang HE
;
Tao ZHANG
;
Dexin YU
Author Information
- Keywords: cisplatin; acute kidney injury; cellular metabolism; glycolysis; amino acid metabolism
- From: Acta Universitatis Medicinalis Anhui 2024;59(5):747-752,760
- CountryChina
- Language:Chinese
- Abstract: Objective To evaluate the change of energy metabolism during cisplatin-induced acute kidney injury.Methods Adult CD-1 male mice were intraperitoneally injected with a single dose of cisplatin (20 mg/kg), and renal function and renal tissue pathology were tested;gene expression was analyzed and signaling pathways were en-riched in cisplatin-treated renal tubular epithelial cells using transcriptome; the contents of renal glycolysis and a-mino acid metabolites were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) .Re-sults Serum urea nitrogen and blood creatinine significantly increased in cisplatin-treated mice.Pathological his-tology observed swelling and shedding of renal tubular epithelial cells.Transcriptome analysis revealed that 2632 genes were upregulated and 2799 genes were downregulated in cisplatin-treated HK-2 cells.GO and KEGG analy-sis showed that differential genes were enriched in energy metabolism.The GSEA analysis results showed that cispl-atin caused an upregulation of the oxidative phosphorylation pathway and a downregulation of the glycolysis pathway in renal tubular epithelial cells, further KEGG analysis demonstrated that cisplatin caused changes in the expression of amino acid genes in renal cells.Metabolomics showed that the contents of glycolytic intermediates and several a-mino acids were altered in the kidney of cisplatin-treated mice.Conclusion Cisplatin-induced acute renal injury is accompanied by modification in renal tubular cell glycolysis and amino acid metabolism.
- Full text:2024062808555023397顺铂致急性肾损伤肾脏糖酵解和氨基酸代谢改变的研究_徐申.pdf
