Changes in renal cell glycolysis and amino acid metabolism during cisplatin⁃induced acute kidney injury
10.19405/j.cnki.issn1000-1492.2024.05.001
- Author:
Shen Xu
1
;
Nannan Liang
2
;
Yahui Ren
1
;
Yizhang He
1
;
Tao Zhang
1
;
Dexin Yu
1
Author Information
1. Dept of Urology, The Second Afiliated Hospital of Anhui Medical University, Hefei 230601
2. School of Public Health , Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
cisplatin;
acute kidney inj ury;
cellular metabolism;
glycolysis;
amino acid metabolism
- From:
Acta Universitatis Medicinalis Anhui
2024;59(5):747-752,760
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To evaluate the change of energy metabolism during cisplatin ⁃induced acute kidney injury.
Methods :Adult CD⁃1 male mice were intraperitoneally inj ected with a single dose of cisplatin (20 mg/kg) , and renal function and renal tissue pathology were tested; gene expression was analyzed and signaling pathways were enriched in cisplatin ⁃treated renal tubular epithelial cells using transcriptome; the contents of renal glycolysis and amino acid metabolites were analyzed using liquid chromatography⁃tandem mass spectrometry ( LC⁃MS/MS) .
Results:Serum urea nitrogen and blood creatinine significantly increased in cisplatin ⁃treated mice. Pathological histology ob served swelling and shedding of renal tubular epithelial cells. Transcriptome analysis revealed that 2 632 genes were upregulated and 2 799 genes were downregulated in cisplatin ⁃treated HK⁃2 cells. GO and KEGG analyatin caused an upregulation of the oxidative phosphorylation pathway and a downregulation of the glycolysis pathway in renal tubular epithelial cells , further KEGG analysis demonstrated that cisplatin caused changes in the expression of amino acid genes in renal cells. Metabolomics showed that the contents of glycolytic intermediates and several amino acids were altered in the kidney of cisplatin ⁃treated mice.
Conclusion :Cisplatin ⁃induced acute renal injury is accompanied by modification in renal tubular cell glycolysis and amino acid metabolism.
- Full text:2024062808555023397顺铂致急性肾损伤肾脏糖酵解和氨基酸代谢改变的研究_徐申.pdf
