Study on the diagnostic value of lymphocyte subpopulations combined with chemokines in children with immunologic thrombocytopenic purpura
10.19405/j.cnki.issn1000-1492.2024.03.027
- Author:
Chentao Shen
1
;
Yalin Xia
1
;
Yeping Sheng
1
;
Peipei Chu
1
;
Jianqin Li
2
Author Information
1. Dept of Hematology , Wujiang Branch of Children Hospital of Soochow University , Suzhou 215200
2. Dept of Hematology , Children Hospital of Soochow University , Suzhou 215000
- Publication Type:Journal Article
- Keywords:
lymphocyte subpopulation;
chemotactic factors;
combined assay;
primary immune thrombocytopenia;
diagnostic efficacy;
ROC analysis
- From:
Acta Universitatis Medicinalis Anhui
2024;59(3):542-546
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the diagnostic value of lymphocyte subpopulations combined with chemokines in children with immunologic thrombocytopenic purpura ( ITP) .
Methods :132 children with proposed diagnosis of ITP were collected , and the children were divided into ITP and non ITP groups according to the diagnostic results of ITP related clinical diagnostic criteria. 6 ml of peripheral venous blood was drawn , the levels of CD4 + CD8 + and CD3 + were detected using flow cytometry , and the levels of chemokine (C-C motif) ligand 5 (CCL5) , Recombi nant Chemokine (C-X-C Motif) Ligand 1 (CXCL11) , and monocyte chemotactic protein 1 (MCP-1) were detec ted using enzyme linked immunosorbent assay , the blood platelet (PLT) was measured by a fully automated cell an alyzer. The children were divided into ITP and non ITP groups according to the clinical diagnostic criteria related to ITP. The lymphocyte subpopulations and chemokine levels of the two groups of children were compared , and the correlation between lymphocyte subpopulations and chemokine levels and PLT was analyzed . The ROC method was used to evaluate the diagnostic efficacy of individual and combined detection of each indicator for ITP.
Results:The levels of CD4 + and CD3 + in the ITP group were lower than those in the non ITP group (P < 0.05) , while the levels of CD8 + were higher than those in the non ITP group (P < 0.05) . The levels of CCL5 , CXCL11 , and MCP-1 in the ITP group were higher than those in the non ITP group (P < 0.05) . The correlation analysis results showed that CD4 + , CD3 + and platelet count were positively correlated in the ITP group(P < 0.05) , while CD8 + , CCL5 , CXCL11 , MCP-1 were negatively correlated with PLT (P < 0.05) . The ROC analysis results showed that the cut off values of CD4 + , CD8 + , CD3 + , CCL5 , CXCL11 , and MCP-1 for the diagnosis of ITP in children were 27.13% , 24.02% , 59.88% , 41.02 ng/L , 30.18 ng/L , and 188.27 ng/L , respectively. The AUC values were 0.893 , 0.880 , 0.629 , 0.801 , 0.892 , and 0.751 , respectively , The AUC of the parallel diagnosis ( meaning that one or more of CD4 + , CD3 + was below the cut off value and/or one or more of CD8 + , CCL5 , CXCL11 , MCP-1 was above the cut off value at the time of parallel testing) was 0.967 , indicating that one or more of them was lower than the cut off value and/or one or more of them was higher than the cut off value when tested separately. Its diag nostic efficacy was higher than that of each indicator tested separately (P < 0.05) .
Conclusion :There are signifi cant differences in lymphocyte subpopulations and chemokines between pediatric ITP patients and non ITP patients . CD4 + , CD8 + , CD3 + , CCL5 , CXCL11 , and MCP-1 can be used for the diagnosis of pediatric ITP. Combined de tection of various indicators can improve detection efficiency.
- Full text:2024062808220967525淋巴细胞亚群联合趋化因子对...性血小板减少症诊断价值研究_沈晨涛.pdf
