Impaired Cation Transport May Lead to Bioelectrical Impedance Changes during Hepatic Ischemia.
10.4174/jkss.2010.78.6.390
- Author:
Mei Lan CUI
1
;
Hyun Soo AHN
;
Jong Yeon KIM
;
Dong Shik LEE
;
Hong Jin KIM
;
Sung Su YUN
Author Information
1. Research Institute of Biomedical Engineering, College of Medicine, Yeungnam University, Daegu, Korea. ssyun@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
Ischemia;
Bioelectrical impedance;
Liver;
Microdialysis;
Cation
- MeSH:
Adenosine Triphosphate;
Electric Impedance;
Electrocardiography;
Extracellular Fluid;
Heart;
Hepatocytes;
Ischemia;
Liver;
Liver Failure;
Microdialysis;
Organothiophosphorus Compounds;
Potassium;
Reperfusion;
Sodium
- From:Journal of the Korean Surgical Society
2010;78(6):390-397
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Ischemia and reperfusion (I/R) injury is a major cause of hepatic failure after liver surgery, but there is no direct method to monitor it in real-time (like an electrocardiogram in heart disease) during surgery. Recently we found the possible role of bioelectrical impedance (BEI) to monitor I/R injury in liver. But the mechanism responsible for ischemia-related BEI changes has not been clearly determined. METHODS: The authors used an LCR meter to quantify BEI changes at 0.12 KHz. Livers were subjected to 70% partial ischemia for 120 minutes, and ATP contents, cation changes in extracellular fluid (ECF; determined using an in vivo intracellular microdialysis technique), hepatocyte sizes, and histological changes were then examined. RESULTS: Liver tissue BEI was found to increase gradually during the first 60 minutes of ischemia and then tended to plateau. During the same period, intracellular ATP contents decreased to below 20% of the baseline level, [Na+] in ECF decreased from 150.4+/-3.8 to 97.8+/-10.6 mmol/L, and [K+] in ECF increased from 7.5+/-0.3 to 34.3+/-5.5 mmol/L during the first 60 minutes of ischemia. Hepatocyte diameter increased by ~20% during the first 60 minutes of ischemia. CONCLUSION: This study suggests that BEI changes during hepatic ischemia are probably caused by sodium and potassium concentration changes in the ECF due to reduced intracellular ATP contents.
