NG2 cell proliferation and activation generate and maintain neuropathic pain in rats after spinal cord injury
10.3760/cma.j.cn115354-20230426-00250
- VernacularTitle:NG2细胞的增殖活化对大鼠脊髓损伤后神经病理性疼痛产生和维持的影响
- Author:
Yehuang CHEN
1
;
Liangfeng WEI
;
Kaiqin CHEN
;
Zhaocong ZHENG
;
Yubei HUANG
;
Jianwu WU
;
Liang XUE
;
Shousen WANG
Author Information
1. 福建医科大学福总临床医学院（解放军联勤保障部队第九〇〇医院）神经外科，福州 350025
- Keywords:
Spinal cord injury;
Neuropathic pain;
NG2 cell;
Chondroitin sulfate proteoglycan
- From:
Chinese Journal of Neuromedicine
2023;22(10):994-1000
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of NG2 cells in generating and maintaining neuropathic pain in rats after spinal cord injury (SCI).Methods:According to random number table method, 100 healthy adult male SD rats were divided into control group ( n=20, without any intervention), sham-operated group ( n=40, exposed T 10 segment without spinal cord impact) and SCI group ( n=40, exposed T 10 segment and constructed SCI model by improved Allen's method). One d before, and 14, 21 and 28 d after surgery, Von Frey fiber probe was used to detect the rat hindlimb mechanical withdrawal threshold (MWT); immunofluorescent staining was used to detect the proportion of NG2-positive cells in spinal dorsal horn cells; Western blotting was used to detect chondroitin sulfate proteoglycan (CSPG) expression in spinal dorsal horn of rats. Results:Fourteen, 21 and 28 d after surgery, SCI group had significantly lower hindlimb MWT, and significantly higher proportion of NG2-positive cells in spinal dorsal horn cells and CSPG expression in spinal dorsal horn than control group and sham-operated group ( P<0.05). One d before, and 14, 21 and 28 d after surgery, in SCI group, hindlimb MWT decreased firstly and increased secondly, proportion of NG2-positive cells in spinal dorsal horn cells increased firstly and decreased secondly, and CSPG expression in spinal dorsal horn increased firstly and decreased secondly. Except for those 21 and 28 d after surgery, hindlimb MWT, proportion of NG2-positive cells in spinal dorsal horn cells, and CSPG expression in spinal dorsal horn showed significant differences between each two time points ( P<0.05). In SCI group, hindlimb MWT was negatively correlated with proportion of NG2-positive cells in spinal dorsal horn cells ( r=-0.876, P<0.001), and CSPG expression was positively correlated with proportion of NG2-positive cells in spinal dorsal horn cells ( r=0.927, P<0.001). Conclusion:NG2 cell proliferation and increased CSPG expression secreted by NG2 cells in spinal cord tissues after SCI generate and maintain neuropathic pain.
