Effect of lactoferrin on biological behaviors of brain glioma cells
10.3760/cma.j.cn115354-20221108-00808
- VernacularTitle:乳铁蛋白对脑胶质瘤细胞生物学行为的影响研究
- Author:
Xianjue WANG
1
;
Gang LIU
;
Liya SU
;
Haibo LIU
Author Information
1. 内蒙古医科大学附属医院临床医学研究中心，内蒙古自治区医学细胞生物学重点实验室，呼和浩特 010050
- Keywords:
Glioma;
Lactoferrin;
Mitochondrial activity;
Cell experiment
- From:
Chinese Journal of Neuromedicine
2023;22(1):51-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of lactoferrin (LF) on biological behaviors of brain glioma cells.Methods:The effect of LF at different concentrations (0, 100, 200 and 300 μg/mL) on the proliferative ability of human glioma cell line U87MG was analyzed by MTT assay to screen the optimal drug concentration. U87MG cells were divided into blank control group and LF (200 μg/mL) treatment group. Edu staining, Transwell assay, Mito-Tracker staining and DCFH-DA staining were used to detect the cell proliferation, migration, mitochondrial activity and reactive oxygen level. Autophagy marker protein microtubule associated protein 1 light chain 3B (LC3B) expression was detected by immunofluorescent chemical staining; contents of superoxide dismutase (SOD) and malonaldehyde (MDA) were detected by colorimetric chemical sensors; expressions of genes related to epithel-to-mesenchymal transformation (E-cadherin, fibronectin, N-cadherin, vimentin and Snail) were detected by reverse transcription (RT)-PCR; expressions of apoptosis-related proteins (Bax and Bcl-2) were detected by Western blotting.Results:Compared with blank control group, the LF treatment group had significantly decreased proportions of Edu positive cells and Mito-Tracker positive cells, number of cell migration and SOD content, and statistically increased proportion of DCFH-DA positive cells, MDA content and LC3B immunofluorescent staining intensity in cytoplasm ( P<0.05). Compared with blank control group, the LF treatment group had significantly decreased E-cadherin mRNA expression and Bcl-2 protein expression, and statistically increased fibronectin, N-cadherin, vimentin and Snail mRNA expressions and Bax protein expression ( P<0.05). Conclusion:LF can effectively inhibit the proliferation, migration, invasion and other biological behaviors of glioma cells, whose mechanism is closely related to mitochondrial activity.
