Uncoupling protein 2 expression and its significance in HT22 cell model with intracerebral hemorrhage
10.3760/cma.j.cn115354-20201209-00956
- VernacularTitle:UCP2在HT22细胞构建的脑出血模型中的表达及意义
- Author:
Peng CHENG
1
;
Datong WANG
;
Zhongwen ZHANG
;
Haibo LU
;
Long YANG
;
Jun LU
;
Deqin GENG
;
Yanbo CHENG
Author Information
1. 江苏省临床重点专科，徐州医科大学公共实验平台，徐州医科大学附属医院神经内科，徐州 221004
- Keywords:
Intracerebral hemorrhage;
Uncoupling protein 2;
Hippocampal neuron;
Oxidative stress;
Dynamic balance of mitochondrial division/fusion.
- From:
Chinese Journal of Neuromedicine
2021;20(11):1101-1107
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the uncoupling protein 2 (UCP2) expression and its relations with apoptosis, oxidative stress and mitochondrial division/fusion in HT22 cell model with intracerebral hemorrhage.Methods:HT22 cells cultured in vitro were divided into control group, and groups of 3, 6, 12 and 24 h after modeling; cells in the later 4 groups were given 10 μmol/L hemin for 3, 6, 12 and 24 h, respectively, and cells in the control group were given the same equivalent solvent for 24 h. The apoptosis of HT22 cells in these 5 groups was detected by Annexin V-FITC/propidium iodide Apoptosis Detection Kit. The reactive oxygen species (ROS) expressions in these 5 groups were detected by fluorescence probe method. Western blotting was used to detect the protein expressions of UCP2, apoptosis-related protein cleaved caspase 3, anti-apoptosis protein B cell lymphoma/leukemia2 (bcl2), and mitochondrial division/fusion proteins (Fis1, Drp1, Opa1, and Mfn2). Results:As compared with the control group, groups of 3, 6, 12 and 24 h after modeling had significantly increased apoptosis rate, ROS level, and UCP2, cleaved caspase 3, Fis1 and Drp1 expressions, and statistically decreased bcl2, Opa1, and Mfn2 expressions. Groups of 3, 6, 12 and 24 h after modeling had successively increased apoptosis rate, successively increased ROS levels, and successively increased UCP2, cleaved caspase 3, Fis1 and Drp1 expressions, and successively decreased bcl2, Opa1, and Mfn2 expressions, with significant differences ( P<0.05). Conclusion:UCP2 expresses in HT22 cell model with intracerebral hemorrhage in time-dependent manner, and it has close relations with apoptosis, oxidative stress, and dynamic balance of mitochondrial division/fusion of HT22 cells.
