Expression of transcription factor Foxp1 in CD4 +T cells of patients with myasthenia gravis
10.3760/cma.j.cn115354-20201204-00946
- VernacularTitle:重症肌无力患者CD4 +T细胞中转录因子Foxp1表达的初步探讨
- Author:
Xiaofang LIU
1
;
Xuejie LI
;
Juan DU
;
Jianping WANG
;
Hong LU
Author Information
1. 郑州大学第一附属医院神经内科，郑州 450003
- Keywords:
Myasthenia gravis;
CD4 +T cell;
Transcription factor Foxp1;
Tacrolimus
- From:
Chinese Journal of Neuromedicine
2021;20(9):886-891
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the transcript factor Foxp1 mRNA expression in peripheral blood CD4 +T cells of patients with myasthenia gravis (MG), and the changes of Foxp1 mRNA expression before and after tacrolimus immunotherapy. Methods:A prospective study was performed. Twenty-six MG patients admitted to our hospital from January 2018 to January 2020 and 18 healthy controls accepted physical examination in our hospital from January 2018 to July 2019 were recruited. Quantitative myasthenia gravis score (QMGs) was used to assess MG severity. Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral venous blood of MG patients before treatment and 6 months after treatment, and healthy controls right after enrollment by density gradient centrifugation; CD4 +T cells were isolated from PBMCs by magnetic cell separation. The Foxp1 mRNA expression was detected by real-time fluorescent quantification PCR (RT-qPCR). Spearman's correlation coefficient was used to evaluate the correlations of Foxp1 mRNA expression with QMGs and serum acetylcholine receptor (AchR) antibody concentration in all subjects. Results:In the 26 MG patients, there were 10 with ocular MG and 16 with general MG; 7 patients were with thymoma, 2 were with thymic hyperplasia, and 17 were with normal thymus. The Foxp1 mRNA expression in CD4 +T cells of MG patients (0.692±0.257) was significantly decreased as compared with that in the heathy controls (1.051±0.364, P<0.05). Moreover, the Foxp1 mRNA expression in general MG patients was significantly lower than that in ocular MG patients ( P<0.05). The Foxp1 mRNA expression in MG patients 6 months after acrolimus monotherapy was significantly increased as compared with that before treatment ( P<0.05). There was a negative correlation between Foxp1 mRNA expression and QMGs ( r=-0.438, P=0.025). Conclusion:Transcript factor Foxp1 mRNA expression is downregulated in CD4 +T cells of MG patients, and its expression is negatively correlated with MG severity, which can reflect the effect of tacrolimus monotherapy on MG to some extent.
