Expression of costimulatory molecule OX40 in peripheral regulatory T cells and its clinical significance in patients with acute cerebral infarction
10.3760/cma.j.cn115354-20200703-00532
- VernacularTitle:共刺激分子OX40在急性脑梗死患者外周血调节性T细胞上的表达及其临床意义
- Author:
Lulu ZHANG
1
;
Cuiping LIU
;
Qi FANG
;
Qun XUE
;
Jianhua JIANG
;
Chunru HAN
;
Juean JIANG
Author Information
1. 苏州大学附属第一医院神经内科，苏州 215031
- Keywords:
Cerebral infarction;
Regulatory T cell;
OX40;
Ischemic brain injury;
Costimulatory molecule
- From:
Chinese Journal of Neuromedicine
2021;20(1):23-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of costimulatory molecule OX40 in peripheral CD4 +CD25 +CD127 low regulatory T (Treg) cells and its clinical significance in patients with acute cerebral infarction (ACI). Methods:Seventy-five patients with first-onset ACI, admitted to our hospital from April 2019 to December 2019, and 36 age- and gender-matched volunteers (control group) were selected in this study. OX40 expression on CD4 +CD25 +CD127 low Treg cells in peripheral blood samples in the two groups were analyzed by immunofluorescent labeling and flow cytometry. Correlations of OX40 +Treg cell percentage with National Institute of Health stroke scale (NIHSS) scores, ischemic penumbra volume, core infarct volume, and infarct volume in the patient group were analyzed. The changes of OX40 +Treg cell percentage in the patient group before and after endovascular treatment or intravenous thrombolysis were compared. Results:As compared with that in the control group, the Treg cell percentage in peripheral blood samples of the patient group was significantly decreased, while OX40 +Treg cell percentage was significantly increased ( P<0.05). OX40 +Treg cell percentage was positively correlated with NIHSS scores in ACI patients ( r s=0.271, P=0.018). Meanwhile, OX40 +Treg percentage was significantly correlated with ischemic penumbra volume, core infarct volume, and infarct volume in the patient group ( r s=0.435, P=0.000; r s=0.343, P=0.003; r s=0.245, P=0.034). OX40 +Treg cell percentage in ACI patients 7 d after endovascular treatment was significantly decreased as compared with that before treatment ( P<0.05); OX40 +Treg percentage in ACI patients 3 and 7 d after intravenous thrombolysis was significantly decreased as compared with that before treatment ( P<0.05). Conclusion:OX40 is abnormally expressed on peripheral Treg cells in ACI patients, and is closely correlated with neurological deficits, imaging features and reperfusion therapy.
