Human urinary kallidinogenase reduces cerebral ischemia-reperfusion injury through activation of phosphatidylinositol 3-kinase/protein kinase B signaling pathway in rats
10.3760/cma.j.issn.1671-8925.2019.05.005
- VernacularTitle:人尿激肽原酶通过激活PI3K/Akt信号通路减轻大鼠脑缺血再灌注损伤
- Author:
Yaowu WANG
1
;
Hongyi ZHANG
;
Chunli YIN
;
Yehua JIA
Author Information
1. 唐山市工人医院神经外科 063000
- Keywords:
Human urinary kallidinogenase;
Cerebral ischemia-reperfusion;
Phosphoinositide 3-kinase/protein kinase B signaling pathway;
Caspase-3
- From:
Chinese Journal of Neuromedicine
2019;18(5):459-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of human urinary kallidinogenase (HUK) on focal cerebral ischemia-reperfusion injury in rats via phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway.Methods Eighty adult male SD rats were randomly divided into sham-operated group,model group,HUK group and LY294002 group (n=20).The rat focal cerebral ischemia-reperfusion models in the latter three groups were established by suture-occluded method;model group and HUK group were,respectively,injected with sterile saline or HUK 1.0 mL/kg via tail vein 3 h after reperfusion;rats in the LY294002 group accepted intraventricular injection of 10 nmol LY294002 before cerebral ischemia and caudal vein injection of 1.0 mL/kg HUK three h after reperfusion.Twenty-four h after reperfusion,Neurological Deficit Scale was performed,cerebral infarct volumes were detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining,and protein expressions of Akt,phosphorylated (p)-Akt and Caspase-3 were assessed by Western blotting.Results As compared with those in the model group,the Neurological Deficit Scale scores were significantly lower,cerebral infarct volumes were significantly smaller,p-Akt expression was significantly increased,and Caspase-3 expression was significantly decreased in the HUK group (P＜0.05).As compared with those in the HUK group,Neurological Deficit Scale scores were significantly higher,infarction volumes were significantly increased,p-Akt expression was significantly decreased,and Caspase-3 expression was significantly increased in the LY294002 group (P＜0.05).Conclusion HUK has neuro-protective effect through up-regulating p-Akt expression in the PI3K/Akt signaling pathway and down-regulating Caspase-3 expression.
