Therapeutic effect of hyperbaric oxygen on hemorrhagic transformation and its mechanism in rats after cerebral infarction
10.3760/cma.j.issn.1671-8925.2019.05.002
- VernacularTitle:高压氧对脑梗死后出血转化的治疗作用及其机制研究
- Author:
Hetao BIAN
1
;
Chunxia DUAN
;
Wei HUANG
Author Information
1. 巨野煤田中心医院神经内科
- Keywords:
Hyperbaric oxygen;
Cerebral infarction;
Hemorrhagic transformation;
Inflammatory factor
- From:
Chinese Journal of Neuromedicine
2019;18(5):442-446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of hyperbaric oxygen on hemorrhage transformation and its possible mechanism in rats after cerebral infarction.Methods Seventy-two SD male rats were randomly divided into sham-operated group,model group,and hyperbaric oxygen group (n=24).Focal cerebral ischemia rat models were established by occluding the middle cerebral artery in model group and hyperbaric oxygen group,and the hemorrhage transformation models after cerebral infarction were prepared by intraperitoneal injection of hyperglycemia before ischemia reperfusion.Rats in the hyperbaric oxygen group were treated with hyperbaric oxygen 3 h after operation.Twenty-four h after operation,Neurological Function Scale was performed;infarct sizes were measured by TTC staining;amount of hemoglobin detected by spectrophotometric assay was used to evaluate the amount of blood loss in the brain tissues;nuclear transcription factor-κB (NF-κB) protein expression was detected by Western blotting;and the concentrations of interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in the penumbra of cerebral infarction were determined by ELISA.Results As compared with those in the model group,the Neurological Function Scale scores of hyperbaric oxygen group were significantly higher (11.50± 1.64 vs.16.04±2.09,P＜0.05).The brain infarct sizes,hemorrhagic volumes,NF-κB protein expression,and IL-1β and TNF-α concentrations in the hyperbaric oxygen group were significantly decreased as compared with those in the model group (33.7％±3.8％ vs.21.6％±4.1％,[11.94±2.26] μL vs.[8.21±1.96] μL,3.05±0.19 vs.2.26±0.25,[49.78±5.65] pg/mL vs.[31.88±2.96] pg/mL,and [31.11± 4.55] pg/mL vs.[19.39±3.19] pg/mL,P＜0.05).Conclusion Hyperbaric oxygen has therapeutic effect on hemorrhage transformation after cerebral infarction by reducing brain infarct sizes and hemorrhagic volumes,whose mechanism might be related to inhibition of NF-κB,IL-1β,and TNF-α expressions and reduce of inflammatory response.
