Effects of low-fat diet or statin intervention at early age on brain Aβ pathology and behaviors of middle-aged Tg2576 mice
10.3760/cma.j.issn.1671-8925.2018.10.002
- VernacularTitle:青、少年期低脂饮食或他汀干预对中年期Tg2576小鼠行为学及脑内Aβ病理的影响
- Author:
Lihua ZHOU
1
,
2
;
Caixia QIU
;
Xuan CHANG
;
Weiming CHEN
;
Jun LIU
;
Zhanhang WANG
;
Wuhua XU
Author Information
1. 510220广州,暨南大学医学院附属广州市红十字会医院康复医学科(神经内科二区),临床病态营养研究所
2. 广东三九脑科医院神经内一科
- Keywords:
Alzheimer's disease;
Atatin intervention;
Low-fat diet;
Amyloid β-protein;
Pathology;
Behavioristics;
Youth;
Juvenile;
Middle age
- From:
Chinese Journal of Neuromedicine
2018;17(10):981-986
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of low-fat diet or statin intervention at early age on brain amyloid β-protein (Aβ) pathology and behaviors of middle-aged Tg2576 mice.Methods Thirty-five two-month-old Tg2576 mice were randomly divided into following 5 groups:a juvenile statin group,a juvenile low-fat diet group,a young statin group,a young low-fat group,and a blank control group (n=7);mice in the low-fat diet groups were given standard low-fat feed,and mice in the statin group were given atorvastatin at 17 mg/(kg· d) into the normal diet.The initiation times of intervention were,respectively,set to be 2-month-old in juvenile groups and 6-month-old in young groups;meanwhile,mice in the blank-control group were fed with normal diet without statin.All mice were raised to be 10-month-old and tested by Morris water maze for evaluating cognitive behaviors two weeks before execution.After peripheral blood and brains being taken,a monoclonal anti-Aβ42 antibody was employed to immunostain mice brain paraffin tissue sections for assaying tissue Aβ plaque immunoreactivity (TAPIR),and the levels of Aβ40,Aβ42,β-secretase,and γ-secretase in homogenates were detected by enzyme-linked immunosorbent assays (ELISA).Results As compared with those in the blank-control group,the average escape latencies,times of passing through hidden platforms,percentage of strong TAPIR,Aβ42 and γ-secretase level in all intervention groups showed no statistical differences (P＞0.05).As compared with those in the blank control group,Aβ42 in homogenates of young intervention groups and β-secretase level in the young statin group were significantly higher (P＜0.05).Conclusion Interventions initiated from juvenile or young,and low-fat diet intervention or statin intervention can neither improve the mice's Morris water maze testing results,nor reduce Aβs burdens in brain homogenates and Aβ40 immunopathologies in brain tissues of middle-aged mice;over early initiation of low-fat diet intervention or statin intervention might accelerate or worsen Alzheimer's disease progress.
